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#Discussion  In an effort to demystify the process of microbial genome sequencing and _ de _de  novo_ assembly, we have designed a workflow that would allow a small lab, one operating without a specialized technician or bioinformatician, to take a sample from swab to publication. There are many options for sequencing, assembling, and annotating microbial genomes. This workflow is only one path through the numerous choices that could be made in a genome sequencing project. All of the scripts and programs for this workflow are open-source and available online for free to ensure that individual researchers and small groups are able to access and utilize the tools necessary to complete the workflow. In general, many available bioinformatic tools are free and open source, but the installation, operation and maintenance of these programs is often complex, requiring specific technical expertise or extensive detailed instructions and best practices in the field remain undefined.