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David Coil edited Library Preparation and Sequencing .md
almost 10 years ago
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#Library Preparation and Sequencing
##Library Preparation
The first choice in library preparation is whether to do the library prep yourself, or to have the library made by your sequencing provider. The economics of this decision are usually dependant on the number of
sample samples involved. For example an Illumina TruSeq library prep kit costs around $2600 for 48 samples. That's far cheaper than the
$??? To $??? $150 to $300 that a typical sequencing provider would charge per sample. However, if you're only preparing a couple of samples there's no reason to buy an entire kit.
Many Most sequencing facilities offer library
preparation. FIND LOCAL PLACE preparation services.
##Kit Options
Whether you chose to make libraries yourself, or use a provider the next major choice is of the type of kit. The two major different choices with Illumina kits are the Nextera transposase-based kits or the TruSeq kits (with or without PCR). These kits are available from Illumina, but there are also comparable options from other vendors (e.g. ??? and ???). The pros and cons of each type of kit are listed below: