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2) are there types of organisms (e.g., pathogens) that you would prefer not to isolate?  - tips -tips  for minimizing pathogen growth-  3) how much time is available for growth and isolation?  - things -things  will grow faster at higher temperatures, but at some point, a threshhold will be reached and many will die -   - you -you  will be able to isolate a greater diversity of organisms if you allow a long time for slow-growing things to grow-  4) what types of equipment are available to you?  - if -if  something grows most happily at 37deg, then you will need to have an incubator and shaker available at that temperature For our previous work we have simply used a rich growth media such as lysogeny broth (LB) (REF)