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Jonathan A. Eisen edited Isolation.md
almost 10 years ago
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#Isolation
##Swab
Using a sterile cotton swab,
swab wipe (i.e., "swab") the area you intend to sample for 10 to 15 seconds, as if you were trying to clean the area. Try to rotate the swab to ensure that all sides touch the surface.
##Plate
Gently (so as not to break the agar surface) streak the swab across the entire surface of an agar plate. Be sure to rotate the swab as you are doing so to ensure that all sides of the swab make contact with the plate. Incubate the plate at the desired temperature
(usually (in our case, usually \(37\,^{\circ}\mathrm{C}\) or room
temperature) temperature - but the desired temperature will depend on the project) for 1-3 days.
##Dilution Streak (streaking for individual colonies) X2
After incubation, choose desired colonies (we typically attempt to maximize the diversity of colony morphologies) and dilution streak them onto individual plates. For a review of dilution streaking technique see for example:
http://www.umsl.edu/~microbes/streakplates.pdf
(THERE SHOULD BE A STABLE REFERENCE INCLUDED HERE TOO OR MORE DETAIL SHOULD BE PROVIDED)
After growth to visible colonies, repeat the dilution streaking to help ensure purity of the culture. Some organisms will only grow in tight association with others, and a mixed culture will prove difficult to work with and almost impossible to sequence/assemble.