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#General notes on Molecular Biology molecular biology  and Microbiology microbiology  This workflow assumes a basic knowledge of molecular biology and sterile technique (methods for carrying out lab experiments without contamination from living microorganisms). The starting point is the collection of microbes from a surface with a swab. We will cover the steps necessary to take a sample through plating, dilution streaking, overnight growth, creating a glycerol stock, 16S rDNA PCR, and preparation for Sanger sequencing to determine the identity of your bacterial or archaeal isolate.