Madison edited 16S rDNA Sequencing and Analysis (Organism Identification).md  over 9 years ago

Commit id: 4f64673b2b2b1215ec37501f62ffc1d74842d1d6

deletions | additions      

       

3. Open the zip file and move the merge\_sanger\_16s.pl file to the new Sanger_seq folder  ###MUSCLE  In order to run this script you will need to download MUSCLE \cite{Edgar_2004} from here: http://www.drive5.com/muscle/downloads.htm. Use the Archive Utility to open the file, change the name of the executable file from something like "muscle3.8.31\_i86darwin64" to "muscle," and move it into your bin directory via the terminal with the following syntax(you syntax (you  will need admin privileges on your machine:sudo cp /Downloads/muscle /usr/bin  sudo cp /Downloads/muscle /usr/bin  ###Convert Files from .abi to . fastq