David Coil edited 16S rDNA Sequencing and Analysis (Organism Identification).md  about 10 years ago

Commit id: 41db5a70977a78d7ebca7dbfbe5b7be48ff203eb

deletions | additions      

       

+"Found N conflicting case(s) during merging of X residues"   or  +"Not enough data to overlap confidently."   In the first case the merging happened however there may be some conflicting bases. The fewer the better. It can be an indication of how confident the user should be with the results. Since this is a very crude method it should be noted that there is no fancy algorithm behind the merge. There is a crude comparison for which we keep the base that had the highest quality score.  The second outcome, the sequences were trimmed too much when doing the QC. The length of both sequences end to end was smaller than the fragment length that we are looking for. This is an indication of poor quality sequence and most users should not proceed (others can lower the quality threshold set by the script).  The newly merged file will be saved as file1_merged.fasta and can be uploaded to BLAST for identification.