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http://www.umsl.edu/~microbes/streakplates.pdf (THERE SHOULD BE A STABLE REFERENCE INCLUDED HERE TOO OR MORE DETAIL SHOULD BE PROVIDED)  After growth to visible colonies, repeat the dilution streaking to help ensure purity of the culture. Some organisms will only grow in tight association with others, and a mixed culture will prove difficult to work with and almost impossible very difficult  to sequence/assemble. ##Liquid Culture  After the second dilution streaking, a liquid culture is needed both for long-term storage and for DNA extraction. Pick a single colony from each dilution streak plate into 5mls of culture media and grow for 1-3 days until cloudy. Once the liquid culture is ready, prepare a 10% final concentration glycerol stock for long-term storage at \(-80\,^{\circ}\mathrm{C}\) from 2mL of the sample.