deletions | additions       diff --git a/Organism Identification using 16s rRNA gene sequence.md b/Organism Identification using 16s rRNA gene sequence.md  index 1f640dd..6fbc6e2 100644  --- a/Organism Identification using 16s rRNA gene sequence.md  +++ b/Organism Identification using 16s rRNA gene sequence.md 
...
It is necessary to screen the 16S rDNA Sanger sequencing results for possible genome sequencing candidates. We recommend starting with BLAST results, then continuing onto the Genomes Online Database (GOLD). This is a large database containing most sequenced genomes and many ongoing sequencing projects. Sometimes the use of GOLD and an internet search will be sufficient to obtain information about the organism you have isolated. In many cases, it will be useful to build a phylogenetic tree to aid in identification, as the BLAST search results may not be sufficiently informative.  ##BLAST 16S rDNA sequence  Begin by navigating to the Standard Nucleotide BLAST [BLAST  at NCBI:  http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome NCBI](http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome)  Paste in your Sanger consensus sequence. We recommend checking the box to exclude Uncultured/environmental sample sequences, since these will not be informative for identification. Be sure the nucleotide collection (nr/nt) is selected under database and click the BLAST button. 
...
http://www.ncbi.nlm.nih.gov/nuccore/  Click on the sequence of interest, then click on the "FASTA" link to get the sequence in FASTA format. Now navigate to the "Align ["Align  Sequences Nucleotide BLAST" page:  http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&BLAST_SPEC=blast2seq&LINK_LOC=align2seq page](http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&BLAST_SPEC=blast2seq&LINK_LOC=align2seq)  Paste in the two 16S rDNA sequences and click on the "BLAST" button. Unless both your sequence and the sequence to which you are comparing were amplified with the same primers, the query coverage will not be 100%. A low identity can be the result of poor sequence quality or taxonomic distance.