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David Coil edited General notes on molecular and microbiology.md
almost 10 years ago
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Throughout this section we refer to "agar" and "culture media". The choice of media will depend on the goals of the particular project. Some factors to consider when selecting media and conditions for growth include:
1.
what What type of organism do you want to isolate?
2.
are Are there types of organisms (e.g., pathogens) that you would prefer not to isolate?
tips for minimizing pathogen growth For example, swabbing people and growing samples on blood agar at 37C is probably not advised in most cases.
3.
how How much time is available for growth and isolation?
+ things will grow faster at higher temperatures, but at some point, a threshhold will be reached and many
will die species won't grow
+ you will be able to isolate a greater diversity of organisms if you allow a long time for slow-growing things to grow
5.
what What types of equipment are available to you?
+ if something grows most happily at
37deg, 37C, then you will need to have an incubator and shaker available at that temperature
For our previous work we have simply used a rich growth media such as lysogeny broth (LB) (REF)