Madison edited 16S rDNA Sequencing and Analysis (Organism Identification).md  over 9 years ago

Commit id: 35f4fc714718f2fd62560a3476bbc7c10f76d60d

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3. Open the zip file and move the merge\_sanger\_16s.pl file to the new Sanger_seq folder  ###MUSCLE  In order to run this script you will need to download MUSCLE \cite{Edgar_2004} from here: http://www.drive5.com/muscle/downloads.htm. Use the Archive Utility to open the file, change the name of the executable file from something like "muscle3.8.31\_i86darwin64" to "muscle," and move it into your bin directory via the terminal with the following syntax (you will need admin privileges on to know  your machine: admin password to do this):  sudo cp /Downloads/muscle /usr/bin