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Madison edited Organism Identification using 16s rRNA gene sequence.md
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##Using GOLD (the Genomes Online Database)
Go to: http://genomesonline.org/cgi-bin/GOLD/index.cgi
Click the search button on the left side of the page and you should be taken to a page that looks like the screen shot displayed in
FigureX. Figure \ref{fig:GOLD}.
Fill out the blue Organism Information (Organism Name) section, with information about your microbe from BLAST and click submit search. We usually search for only the genus to get a sense for how well that genus is represented in the database and which species are present. Figure
10 \ref{fig:GOLD\_results} shows an example screen shot of the results for "Brachybacterium." Clicking on a project ID will take you to a
omre more detailed description of the project including its project status (complete, permanent draft, incomplete, targeted).
If you have relatively ambiguous identification results (e.g. you think you have some sort of Brachybacterium but aren't sure which species) it could be worthwhile to perform an alignment of your 16S sequence with those from genomes already in Genbank.