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David Coil edited Organism Identification using 16s rRNA gene sequence.md
almost 10 years ago
Commit id: 22d110b8485b15533da0e37ee657f95ba2304445
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##BLAST 16S rDNA sequence
Begin by navigating to the Standard Nucleotide BLAST at NCBI:
http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE\_TYPE=BlastSearch&LINK_LOC=blasthome http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome
Paste in your Sanger consensus sequence. We recommend checking the box to exclude Uncultured/environmental sample sequences since these will not be informative for identification. Be sure the nucleotide collection (nr/nt) is selected under database and click the BLAST button.