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8. Click on "Do Classification With Selected Sequences" button. This will show you a hierarchical view of the classification of your sequence (from Phylum to Genus.) You will use this information to navigate to other sequences that you want to include in your alignment that you will use to build your phylogenetic tree. For example, Figure \ref{fig:RDP} shows the hierarchy for the _Tatumella_ 16S sequence.   8. Click on BROWSERS. We recommend opening BROWSERS in a new tab so that you can keep the hierarchy information handy.  9. Click on "Isolates" to select only isolates for further analysis. Then click "Browse"  10. Click on the + sign next to "Archaea outgroup." This will add an Archaeal sequence to your cart, which will be used to root your phylogenetic tree. Even better would be to chose an outgroup within the same bacterial phyla that you know to be outside of the clade you are examining. If in doubt, just use the Archaeal one.  11. If using the example sequence provided, click on "Proteobacteria", then Gammaproteobacteria, then "Enterobacteriales", then Enterobacteriaceae. This will take you to the Genus Tatumella, which currently has over 69 entries in it. If the genus you are working with has too many sequences to analyze easily (for example, _Bacillus_ currently has >26000,) one way to reduce this number is to exclude the uncultured taxa in the database. To do this, scroll down to the Data Set Options and click on the "Isolates" button. Click "Refresh" and you will see that there are fewer sequences in the Genus. To reduce this number further, click on the "Type" Strain button (though if you do this you'll have to build a tree later for species identification since each species will only be represented once in the tree). As a worst-case scenario, you will need to manually select a subset of organisms to include in your alignment.  12. Click on the + sign next to **genus** _Tatumella_ to add all of those sequences to your cart.  13. Click on "Sequence Cart" and confirm that your uploaded sequence, the outgroup sequence, and all of the other sequences you'd like to include in your tree are displayed.