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This figure shows the estimated materials (i.e. without labor) cost of performing a genome sequencing project. project with this workflow.  The "Best Case" shows the marginal cost of sequencing onemore  genome in a case where you are multiplexing 48 samples, and have the appropriate kits and reagents on hand. The "Worst Case" shows the cost of doing a single genome, with no multiplexing, in a lab where every reagent needed to be purchased new and was not used for anything else. Specific assumptions are as follows; 1This assumes the purchase of a standard DNA extraction kit, good for 100 samples. 2This assumes purchase of a standard 200U PCR reagent kit. 3PCR cleanup can be performed in a number of ways; gel extraction, beads, or columns for example. Here we assume purchase of a standard column-based kit. 4Sanger sequencing cost is given as the price per reaction ($7 at our sequencing facility), times the forward and reverse reactions. 5This assumes the purchase of a 48-sample Nextera or TrueSeq kit from Illumina, however kits from other manufacterers can be cheaper. 6Our sequencing cost estimate assumes to purchase of an Illumina MiSeq run from a sequencing facility.