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Jonathan A. Eisen edited Organism Identification using 16s rRNA gene sequence.md
almost 10 years ago
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1. In FigureX, it is not necessary to build a phylogenetic tree for further identification. All of the top hits are either Kocuria rosea or Kocuria sp, have e-values of 0.0, perfect query coverage, and 99% to 100% identity. In this case, proceed to "Using GOLD".
2. In FigureX the results are much more ambiguous. The results show more than 99% identity to multiple species within multiple genera. In this case, proceed to "Making a Phylogenetic Tree", before using GOLD.
##Using GOLD
(the Genomes Online Database)
Go to: http://genomesonline.org/cgi-bin/GOLD/index.cgi
Click the search button on the left side of the page and you should be taken to a page that looks like the screen shot displayed in FigureX.