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David Coil edited 16S rDNA Sequencing and Analysis (Organism Identification).md
almost 10 years ago
Commit id: 02418b7fe104858700737ef07efaf8c6d4052b56
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##Direct PCR (if not extracting DNA)
Centrifuge 1mL of the overnight culture until the bacteria form a pellet at the bottom of the tube (about 5 minutes at 10,000g), pour off the supernatant and resuspend in
100\(mu) ul 100ul of sterile DNAase-free water. Incubate the samples at 100°C for 10 minutes to help lyse the cells. Use the resulting solution as the template in the PCR reaction below
##PCR reaction
This reaction uses the 27F (AGAGTTTGATCMTGGCTCAG) and 1391R (GACGGGCGGTGTGTRCA) primers which amplify a near full-length bacterial 16S rRNA gene. Our lab uses either the ___ or the ___ PCR reagents, with an annealing temperature of \(54\,^{\circ}\mathrm{C}\) and an extension at \(72\,^{\circ}\mathrm{C}\) of 90 seconds. Don't forget to include positive (any sample containing bacterial genomic DNA) and negative (water) controls.