Jenna M. Lang edited Introduction.md  over 9 years ago

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An ecological perspective helps to delineate the complexity and multi-layered nature of the relationships between the microbiota, the human host, and both the nutritive and non-nutritive compounds we ingest \cite{Costello\_2012}. The concept of the human gut microbiome as a distinct ecosystem allows us to identify and characterize the components of the system, including its inputs and outputs. In this case, the inputs of the system include all of the various ingested compounds that can either serve as food substrates (e.g. complex sugars) or that can be metabolized by or that affect the metabolism of the microbiota (e.g. polyphenolic compounds, environmental chemicals, medications). Some of these inputs, such as the microbial food substrates (i.e. prebiotics) have been studied extensively. It has been well documented that certain sugars such as galactooligosaccharides, fructooligosaccharides, and oligosaccharides found in milk act as prebiotics that support the establishment and growth of certain commensal microbial species \cite{Brownawell\_2012,Vrese\_2008, Roberfroid\_2008, German\_2008, Zivkovic\_2011}. Research has also documented the effects of antibiotics, and pathogens on the microbiota composition, its recovery or lack of recovery to baseline following resolution, and the various immunological and physiological effects of these \cite{Manichanh\_2010, Ubeda\_2012,Bien\_2013}.   Yet there is little information on the effects of ingested microorganisms that are present in our diets on gut microbiota composition or function, and even the basic questions of which microbes, how many of them, and how much they vary from diet to diet and meal to meal, have not been answered. What is known is the microbial ecology of various specialty foods where fermentation, colonization, ripening, and/or aging are part of the preparation of these foods, for example pancetta \cite{Busconi_2014} and of course cheese \cite{Gatti_2008}. The microbial ecology of the surfaces of raw plant-derived foods such as fruits and vegetables has also been characterized \cite{Leff_2013}. \cite{23544058}.  There is a large base of literature on food-borne pathogens \cite{Aboutaleb_2014}. Furthermore, it is known that the microbial ecology of endemic microbes found on food surfaces can affect mechanisms by which pathogens colonize these foods \cite{Critzer_2010}. A recent article showed that certain ingested microbes found in foods such as cheese and deli meats were detected in the stool of individuals who consumed them, and that furthermore they were culturable and thus survived transit through the upper intestinal tract \cite{David_2013}. However, the basic microbial ecology of different meals and diets, as well as the total number of live microorganisms ingested in these meals and diets are largely unknown. We hypothesized that the microbes we eat vary both quantitatively and compositionally in a significant way according to dietary pattern. We have selected to characterize the microbiota of three different dietary patterns in order to determine: the average total amount of daily microbes ingested via food and beverages and their composition in the average American adult across three different dietary patterns: 1) the Average American dietary pattern (AA) focused on convenience foods, 2) the USDA recommended dietary pattern (USDA) emphasizing fruits and vegetables, lean meat, dairy, and whole grains, and 3) the Vegan (VEG) dietary pattern, which excludes all animal products. Here we used DNA sequencing, culture counting, and informatics methods to characterize microbes in these dietary patterns.