Jenna M. Lang edited Methods.md  about 10 years ago

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#Sample Collection  The detailed meal plans with all ingredients are shown in Table 1, food preparation descriptions (all steps prior to placing into blender and blending foods as described in methods section) are shown in Table 2, and nutrient values based on the USDA nutrient database are shown in Table 3. The AA meal plan consisted of a large Starbucks Mocha Frapuccino for breakfast, a McDonald’s Big Mac, French fries, and Coca Cola for lunch, Stouffer’s lasagna for dinner, and Oreo cookies for a snack. The USDA meal plan consisted of cereal with milk and raspberries for breakfast, an apple and yogurt for a morning snack, a turkey sandwich on whole wheat bread with salad (including a hard-boiled egg, grapes, parmesan cheese, and Ceasar dressing) for lunch, carrots, cottage cheese and chocolate chips for an afternoon snack, and chicken, asparagus, peas and spinach on quinoa for dinner. The VEG meal plan consisted of oatmeal with banana, peanut butter, and almond milk for breakfast, a protein shake (including vegetable-based protein powder, soy milk, banana and blueberries) for a morning snack, a vegetable and tofu soup (including soba noodles, spinach, carrots, celery and onions in vegetable broth) for lunch, an apple and almonds with tea for an afternoon snack, a Portobello mushroom burger (including Portobello mushroom, avocado, tomato, lettuce, and a whole wheat bun) with steamed broccoli for dinner, and popcorn, hazelnuts and fig bars for an evening snack.   The following meals contained fermented foods that contained live active cultures according to the package and were consumed without heat treatment: USDA meal plan snack #1 (yogurt), lunch (parmesan cheese), and snack #2 (cottage cheese). The following meals contained fermented foods that were cooked as part of meal preparation: VEG meal plan lunch (tofu), and AA meal plan lunch and dinner (cheese). Meal ingredients were purchased at local grocery stores in Saint Helena, CA, and prepared meals were purchased in restaurants in Napa, CA.  #Survey of Microbial Community Composition  #Statistical Analyses