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Gold nanoparticles patterned using DNA brick crystals. a–c, Model (a)and TEM images (b,c) of parallel lines of 10 nm gold nanoparticles closely packed on a ZX-4H× 6H× 96-channel crystal. c, Zoomed-in TEM image of a single chain of gold nanoparticles. d–f, Model (d)and TEMimages (e,f) of close-packed gold-nanoparticle monolayers formed on the top and bottom surfaces of an XY-4H× 4H× 64B-cuboid crystal. Inset of d: single-stranded poly-T extensions on each end of the helix and a 10 nm gold nanoparticle occupying a 4H× 4H surface. A crystal display curvature on the edge (f). Pink arrows indicate the curved positions where the two gold-nanoparticle monolayers can be seen. [31]  And despite templated-guided self-assembly based on EBL and lift-off process, the nanoparticle’s position can also be tuned by properly designed and functionalized DNA origami. [29,30] That by proper designing the base sequence of the DNA strand, the resulting origami structure’s shape can be well defined. And the nanoparticle can be attached to the DNA origami by proper modification of certain DNA strand. Compared to other method to assemble nanoparticles, DNA origami has higher resolution down to 2 nm (the diameter of each helix is ~2 nm). (Fig. 25)\\