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  %\subsection{Data processing}  The frames containing single photon events were processed with the ThunderSTORM \cite{Ovesny2014} superresolution imaging plug-in for ImageJ. Due to memory limitations, the USAF data was processed in 6$\times$5,000 and the cell data in 3$\times$2,000 image stacks. The software first detects the events from the noise background, and an approximate localization algorithm locates due to  thecenter pixel of each event. The  peaked pulseheight distribution of the photon events \cite{Hirvonen2014_rsi}makes  the setting of a threshold is  relatively straight-forward. A An approximate localization algorithm locates the center pixel of each event, and a  sub-pixel localization algorithm then calculates the center of the events with greater resolution. within the pixel.  The software camera parameters were set to 80.0~nm pixel size and 36 photoelectrons per A/D count. The base level varied between image stacks due to fluctuations in the EBCCD temperature, and was set to the average minimum grey value for the image stack in the range of 100-140 A/D counts. A wavelet (b-spline) image filter was applied with order of 3 and scale of 2.0. For the approximate localization of the events, the  centroid of connected components method was used with a peak intensity threshold (PIT) of 2*std(Wave.F1) for the USAF data, and a PIT of 1.5*std(Wave.F1) for cell data, with the  watershed algorithm enabled for all data. Maximum Likelihood (ML) fitting was used with a  Gaussian point-spread function (PSF), with standard deviation (SD) set to 1.0 pixels. For fast processing with adequate results the PSF fitting radius was set to 2 pixels, and for optimal photon detection and separation of overlapping events the radius was set to 7 pixels. Multiple-emitter fitting analysis (MFA) was tested with a maximum of 2 molecules per fitting region with a model selection threshold (p-value) of 10$^{-6}$. When MFA was enabled, ThunderSTORM's ``remove duplicates'' post-processing tool was applied with a distance threshold of 160~nm, and ``intensity$>$4000'' filter was applied to the USAF data and ``intensity$>$3000'' filter to the cell data. Least A least  squares (LS) fitting method was also tested with an  integrated Gaussian (IG) PSF with a  3 pixel fitting radius and 1.6 pixel SD, and a  radial symmetry localisation method with 2 pixel estimation radius.