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ThunderSTORM's option for Multi-emitter Fitting Analysis (MFA) produces excellent results with recognising and separating overlapping EBCCD photon events, as shown in Fig~\ref{fig_mfa}. For the USAF data containing an average of 150 photons/frame the photon count increases 2\% with MFA enabled.  Centroiding was then applied to higher photon density biological cell fluorescence microscopy  data containing of a fixed cell labelled with xxxx. The frames contain  an average of 460 photons/frame (Fig~\ref{fig_cell}). With 7-pixel PSF fitting radius and MFA enabled, the photon count increases 13.0\% compared to using 2-pixel fitting radius, and 5.2\% compared to 7-pixel fitting radius without MFA. Fitting of multiple PSFs is time-consuming and the processing time increases significantly with MFA enabled, as expected (see Table~\ref{table:results}). However, for biological imaging the image acquisition time is a critical parameter, and the separation of overlapping events can potentially lead to significant reduction in image acquisition times.