Liisa Hirvonen edited Results: Events.tex  almost 9 years ago

Commit id: b543548e205946abd8726388937d5c34ca7ab998

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The ion events cause problems with event recognition algorithms that find a threshold for each frame separately: the high brightness causes the threshold to be set too high and the photon events discarded as noise. The raw data was therefore preprocessed using ImageJ's tools by setting the intensity of all bright pixels in the ion events to a grey value slightly above the maximum intensity of the photons events. The ion events are then incorrectly localised as photon events, but due to the relatively rare occurrence of ion events compared to photon events (around 1 ion event per 600 photon events) this does not have a noticeable effect on the results.  *** Something about the event recognition methods and how well they work with our data... *** For the MR processing, the enabling of MFA with a maximum of 2 molcules per fitting region introduces a bias into the algorithm that it should anticipate detecting 2 photon events per fitting region. This can initially cause single photon events to be over-resolved into multiple events. The occasions where single events are over-resolved is counteracted by the remove duplicates function; as the over-resolved detection markers are within nanometres of each other (within the same pixel) the excess marker can be removed whilst not affecting correctly-resolved overlapping events, which are of the order of micrometres apart.