deletions | additions       diff --git a/Method.tex b/Method.tex  index ce214a3..0836ada 100644  --- a/Method.tex  +++ b/Method.tex 
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For the sub-pixel localization algorithms, integrated Gaussian (IG) PSF was set to a 3 pixel fitting radius, a 1.6 pixel standard deviation and the least squares (LS) fitting method and radial symmetry was applied with a 2 pixel estimation radius. To achieve good photon detection with adequate FPN in a very short period of time, the data can be processed with the Fast Approximation (FA) settings described below. To achieve optimal photon detection, the resolution of overlapping events and a lower FPN, the Maximum Resolution (MR) settings can be applied to the data, however this produces a substantial increase in processing time. For the FA processing approximate localization algorithms, Gaussian PSF was set to a 2 pixel fitting radius with a 1.0 pixel standard deviation and the Maximum Likelihood (ML) fitting method, and for the MR processing, Gaussian PSF was set to a 7 pixel fitting radius with a 1.0 pixel standard deviation with the Maximum Likelihood (ML) fitting method.   When the multiple-emitter fitting analysis (MFA) was applied in conjunction to the PSF gaussian or IG algorithms, it was used with a maximum of 1 molecule per fitting region and a model selection threshold (p-value) of 10^{-6} for the FA processing, or a maximum of 2 molecules per fitting region and a model selection threshold (p-value) of 10^{-6} for the MR processing. For USAF test pattern data processed using the MR settings with MFA enabled, thunderSTORM's remove duplicates post-processing tool was applied with a distance threshold of 160~nm and an intensity>4000 filter 'intensity>4000 filter'  was used. For cells data, the remove duplicates tool was also applied with a distance threshold of 160~nm but with an intensity>3000 'intensity>3000'  filter. No post-processing was required for data processed using FA settings.