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Liisa Hirvonen edited Introduction.tex
almost 9 years ago
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Single-molecule localization microscopy is now a well-established technique, and a variety of free software is available for image reconstruction. As the usefulness of this method crucially depends on both the localization accuracy and precision, much effort has been put into the development and optimization of many different types of centroiding algorithms, including iterative fitting algorithms. As recently reported, super-resolution software for single-molecule localisation gives good results when applied to centroiding single photon events imaged with an MCP-intensifed CMOS camera.\cite{Hirvonen2015_OL} In this work, we have applied super-resolution software for centroiding single photon events events detected with an EBCCD camera. Multi-emitter fitting analysis was also tested for separating overlapping photon events, an important aspect which allows an increased count rate and shorter acquisition times.
Test edit.