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A submicrowatt excitation power over the whole field of view is sufficient for this approach, and compatibility with live-cell imaging was demonstrated by imaging europium-containing beads with a lifetime of 570\&\#xA0;\&\#x3BC;s in living HeLa cells. A standard two-photon excitation scanning fluorescence lifetime imaging (FLIM) system was used to independently verify the lifetime for the europium beads. This approach brings together advantageous features for time-resolved live-cell imaging such as low excitation intensity, single-photon sensitivity, ultrafast camera frame rates, and short acquisition times.}, Doi doi  = {10.1364/OL.39.005602}, Keywords keywords  = {Photon counting; Low light level; Microscopy; Fluorescence microscopy; Lifetime-based sensing; Time-resolved imaging}, Publisher publisher  = {OSA} {OSA},  }  @Article{Hirvonen2015_nima,  Title = {Photon counting imaging with an electron-bombarded \textsc{CCD}: towards wide-field time-correlated single photon counting (\textsc{TCSPC})},  Author = {Liisa M. Hirvonen and Stephen Jiggins and Nicolas Sergent and Gianmarco Zanda and Klaus Suhling},  Journal = {Nucl Instrum Meth A},  Year = {2015},  Volume = {doi:10.1016/j.nima.2015.01.031},  Doi @Article{Hirvonen2015_nima,  title  = {10.1016/j.nima.2015.01.031} {{Photon counting imaging with an electron-bombarded \textsc{CCD}: towards wide-field time-correlated single photon counting (\textsc{TCSPC})}},  author = {Liisa M. Hirvonen and Stephen Jiggins and Nicolas Sergent and Gianmarco Zanda and Klaus Suhling},  journal = {Nucl Instrum Meth A},  year = {2015},  volume = {doi:10.1016/j.nima.2015.01.031},  doi = {10.1016/j.nima.2015.01.031},  }  @Article{Hirvonen2014_rsi,  Title title  = {Photon {{Photon  counting imaging with an electron-bombarded \textsc{CCD}: towards a parallel-processing photoelectronic time-to-amplitude converter},  Author converter}},  author  = {Liisa M. 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Hirvonen and Zden\v{e}k Petr\'{a}\v{s}ek and Klaus Suhling},  journal = {Nucl Instrum Meth A},  year = {2014},  volume = {doi:10.1016/j.nima.2014.09.082},  doi  = {10.1016/j.nima.2014.09.082}, ISSN issn  = {0168-9002} {0168-9002},  }  @Article{Hosny2012,  Title title  = {Single {{Single  photon counting fluorescence lifetime detection of pericellular oxygen concentrations},  Author concentrations}},  author  = {Hosny, N. A. and Lee, D. A. and Knight, M. M.}, Journal journal  = {J Biomed Opt}, Year year  = {2012}, Number number  = {1}, Pages pages  = {016007}, Volume volume  = {17} {17},  }  @Article{Hosny2010,  Title title  = {Extracellular {{Extracellular  oxygen concentration mapping with confocal multiphoton laser scanning microscope and TCSPC card},  Author card}},  author  = {Hosny, N. A. and Lee, D. A. and Knight, M. 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