Quick pilot experiment with Luis’ positive control plastic (tube 1) and “Foil + EtOH + fire #2” plastic sample (tube 3) added to 5mL ACES-buffered yeast extract broth supplemented with FeNO3, L-cys and thymidine (standard culture medium for Legionella pneumophila studies. ph = 6.9 (blank tube 2)

After 24h on roller at 37C, no growth was observed. Examination under light microscope reveals only bacteria in positive control. Returned to wheel for the weekend.