10ul of each AYE tube (positive control, PLA plastic and negative control) was struck out on Charcoal Yeast Extract solid media and put at 37C to grow for 24h. Growth revealed that the PLA tube (top, white) appeared to contain a different bacterial species than the positive control tube (bottom, yellow). The negative control was plated on the right (no growth). Under light microscope, both bacterial growths appear coccoid, with the yellow colonies forming clumps more often. The tube experiment was repeated and set up including parts from Luis and Russell, plus controls. So far, no growth observed for any 3D parts at 24h.