Kaitlin Flynn edited Methods1.tex  over 10 years ago

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\section{Methods}  \subsection{1) Bacterial strains, culture conditions and reagents}  \begin{itemize}  \item For AYE growth experiments, 3D parts were cultured on a rolling spinner  at 37°C in N- (2-acetamido)-2- aminoethanesulfonic acid (ACES; Sigma)-buffered yeast extract (AYE) broth supplemented with 100μg/ml thymidine (Sigma). To quantify colony forming units (CFU), aliquots were plated on ACES-buffered charcoal-yeast extract agar (CYE) supplemented with 100μg/ml thymidine (T) and incubated at 37°C for 4 days. \item Terrific Broth (TB) experiments were conducted on a rolling spinner at 37°C  in media containing yeast extract, tryptone and glycerol supplemented with 0.17 M KH_2PO_4, 0.72 M K_2HPO_4 K_2HPO_4.   \item Meat broth experiments were performed in media containing yeast extract, beef extract, glucose, maltose, fructose, xylose in an anaerobic chamber at 37°C.  \end{itemize}