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Kaitlin Flynn edited Methods1.tex
over 10 years ago
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\section{Methods}
\subsection{1) Bacterial strains, culture conditions and reagents}
\begin{itemize}
\item For AYE growth experiments, 3D parts were cultured
on a rolling spinner at 37°C in N- (2-acetamido)-2- aminoethanesulfonic acid (ACES; Sigma)-buffered yeast extract (AYE) broth supplemented with 100μg/ml thymidine (Sigma). To quantify colony forming units (CFU), aliquots were plated on ACES-buffered charcoal-yeast extract agar (CYE) supplemented with 100μg/ml thymidine (T) and incubated at 37°C for 4 days.
\item Terrific Broth (TB) experiments were conducted
on a rolling spinner at 37°C in media containing yeast extract, tryptone and glycerol supplemented with 0.17 M KH_2PO_4, 0.72 M
K_2HPO_4 K_2HPO_4.
\item Meat broth experiments were performed in media containing yeast extract, beef extract, glucose, maltose, fructose, xylose in an anaerobic chamber at 37°C.
\end{itemize}