deletions | additions       diff --git a/Methods1.tex b/Methods1.tex  index badad01..ae61284 100644  --- a/Methods1.tex  +++ b/Methods1.tex 
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1) \textbf{1)  Bacterial strains, culture conditions and reagents. reagents.}  \begin{itemize}  \item For AYE growth experiments, 3D parts were cultured at 37°C in N- (2-acetamido)-2- aminoethanesulfonic acid (ACES; Sigma)-buffered yeast extract (AYE) broth supplemented with 100μg/ml thymidine (Sigma). To quantify colony forming units (CFU), aliquots were plated on ACES-buffered charcoal-yeast extract agar (CYE) supplemented with 100μg/ml thymidine (T) and incubated at 37°C for 4 days.  \item  \end{itemize}