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\subsection{1) Bacterial strains, culture conditions and reagents}  \begin{itemize}  \item For AYE growth experiments, 3D parts were cultured at 37°C in N- (2-acetamido)-2- aminoethanesulfonic acid (ACES; Sigma)-buffered yeast extract (AYE) broth supplemented with 100μg/ml thymidine (Sigma). To quantify colony forming units (CFU), aliquots were plated on ACES-buffered charcoal-yeast extract agar (CYE) supplemented with 100μg/ml thymidine (T) and incubated at 37°C for 4 days.  \item Terrific Broth (TB) experiments were conducted in media containing yeast extract, tryptone and glycerol supplemented with 0.17 M KH2_expPO_exp, 0.72 M K2HPO4  \end{itemize}