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Kaitlin Flynn added Methods1.tex
over 10 years ago
Commit id: 404f5b0649c615aa08c04b97bad3d2e0e299f2e3
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1) Bacterial strains, culture conditions and reagents.
\begin{itemize}
\item For AYE growth experiments, 3D parts were cultured at 37°C in N- (2-acetamido)-2- aminoethanesulfonic acid (ACES; Sigma)-buffered yeast extract (AYE) broth supplemented with 100μg/ml thymidine (Sigma). To quantify colony forming units (CFU), aliquots were plated on ACES-buffered charcoal-yeast extract agar (CYE) supplemented with 100μg/ml thymidine (T) and incubated at 37°C for 4 days.
\end{itemize}
