deletions | additions       diff --git a/Reproduction of growth experiment.tex b/Reproduction of growth experiment.tex  index 08d0e2a..908a8cf 100644  --- a/Reproduction of growth experiment.tex  +++ b/Reproduction of growth experiment.tex 
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\subsection{Independent reproduction of growth experiment on printed component}  \label{sec:indpendent_rep}  The above experiment using printed components was replicated at Michigan State University. We used a similar kit-built Ultimaker 3D printer modified with an E3D all metal print head with a 0.4mm nozzle \cite{http://e3d-online.com/}. A cylindar was designed using OpenSCAD with a radius of 4mm and a height of 12mm. The model was exported to an STL object and sliced with Cura SteamEngine 13.12. The cylinder was printed with a wall thickness of 0.4mm, a feed-rate of 10mm/second (the effective speed with the minumum layer cooling time set to 5 seconds), and a nozzle temperature of $225^{\circ}C$. $225^{\circ}\mathrm{C}$.  The printbed was prepared with 3M Scotch Blue painters tape and was lightly wiped with ethanol before printing began. Two printed cylinders were transfered to sterile glass tubes filled with 4mL of Luria-Burtani (LB) broth with flamed tweezers. A fragment of unused fillament was used as a positive control, and an uninoculated tube was used as a negative control. Tubes were transfered to a shaking incubator set at $30^{\circ}C$. $30^{\circ}\mathrm{C}$.  No growth was observed after 24 hours in any of the tubes with printed parts, while the unused fillament contaminated the media. After two days, another cylinder was printed and incubated in LB broth. Again, after 24 hours no growth was observed. None of the tubes with printed parts showed signs of growth after 96 hours.