deletions | additions       diff --git a/Introduction.tex b/Introduction.tex  index 137478f..efdc3fd 100644  --- a/Introduction.tex  +++ b/Introduction.tex 
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\begin{itemize}  \item Test for growth on richer media than LB :  \begin{itemize}  \item blood media Blood, serum, chocolate agar  \item SOB  \item AYE (ACES Yeast Extract broth)   \item grow with CO2, in "candle can" + anaerobically   \item stimulate w/ known germinants to induce spore germination   \item let sit in broth on bench for a few weeks, then plate for CFU  \end{itemize}  \item Plate onto  \begin{itemize}  \item LB  \item blood agar  \item fungal media (Sabouraud?)  \item chocolate, maconkey, TSA, etc agar (See above)   \item test if leeching from or PLA itself affects tissue cell culture growth, both cell lines and bone marrow derived macrophages   \item see if potential microbes can be resusitated in amoebic or other hosts  \end{itemize}  \item See if this technique can possibly be compliant with CLIA (Emily's suggestion)  \end{itemize}