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The utility of decomposing the morphological variation in its symmetric and asymmetric components in order to elucidate differences between groups has not been sufficiently explored. Previous studies show the adequacy of this approach to detect specific patterns of morphological variation that could even be useful to taxonomically discriminate species within a genus. This methodology has only been used to study the morphology of taxa with fractal and radial symmetry.  In order to further explore the importance of shape variation for the morphological delimitation and identification of \textit{Carollia} species, in this study, it was aimed to isolate and study the patterns of symmetric and asymmetric morphological variation of the genus \textit{Carollia} in Colombia.