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Before extraction, each plant was cut into roughly 50 pieces. The first plant was cut into forty-six pieces (summarized in the figure below). Previous data (unpublished) had shown obvious differences between tissue types, so our study focused on fine-scale community variation within each tissue type rather than among them. Briefly, leaves were cut into twenty roughly equally-sized pieces (about five centimeters each). The relative position of each piece was recorded. Similarly, the rhizome was sectioned into six segments. Each rhizome segment was between 1.5 and 2 centimeters in length and contained no more than a single root bundle.  Roots Because we were unsure of the appropriate amount of tissue for extraction, half of the roots were sectioned into four smaller pieces while the other half were left whole.