Steven Roberts edited Introduction.md  about 10 years ago

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#Introduction  Over the past several years our lab has experimented with a variety of high-throughput sequencing platforms with the ultimate goal of answering an addressing  important scientific question. questions.  On a few occasions we have been successful. Below is a table of some of these products.    Goetz FW, Rosauer D, Sitar S, Goetz G, Simchick C, Roberts SB, Johnson R, Murphy C, Bronte C, Mackenzie S. (2010) A genetic basis for the phenotypic differentiation between siscowet and lean lake trout (Salvelinus namaycush). Molecular Ecology, 19 176–196  Seeb JE, Pascal CE, Graue ED, Seeb LW, Templin WD, Harkins T, Roberts SB. (2010) Transcriptome sequencing and high-resolution melt analysis advance single nucleotidepolymorphism discovery in duplicated salmonids. Molecular Ecology Resources. doi: 10.1111/j.1755-0998.2010.02936.x  Morera D, Roher N, Ribas L, Balasch JC, Doñate C, Callol A, Boltaña A, Roberts SB, Goetz G, Goetz FW, Mackenzie SA. (2011) RNA-Seq reveals an integrated immune response in nucleated erythrocytes. PLoS ONE 6(10): e26998. doi:10.1371/journal.pone.0026998  Gavery MR* and Roberts SB. (2012) Characterizing short read sequencing for gene discovery and RNA-Seq analysis in Crassostrea gigas. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 7:2 94-99 June 2012. doi:10.1016/j.cbd.2011.12.003  Roberts SB, Hauser L, Seeb LW, Seeb JE (2012) Development of genomic resources for Pacific herring through targeted transcriptome pyrosequencing. PLoS ONE 7(2): e30908. doi:10.1371/journal.pone.0030908  Burge CA, Douglas N, Conti-Jerpe I, Weil E, Roberts SB, Friedman CS, CD Harvell. (2012) Friend or foe: the association of Labyrinthulomycetes with the Caribbean sea fan, Gorgonia ventalina. Diseases of Aquatic Organisms. 101:1-12 doi:10.3354/dao02487    Table? links   Table  Project | Raw | Reference Gavery MR, Roberts SB. (2013) Predominant intragenic methylation is associated with gene expression characteristics in a bivalve mollusc. PeerJ 1:e215 doi:10.7717/peerj.215      However there are a number of efforts that have failed early on (ie poor read quality) or just never gained enough momentum to see the light of a the peer-review system. This paper is a final attempt to share these datasets in a manner where they have the potenial, however slim, to be useful to someone. We will not be providing fluffy background or in-depth over-analysis but strive to offer a sound 'methods' and 'results' section if you are consider a conventional paper, which this not. This current work will be organized by tareget species, which will often correspond to progject.