Steven Roberts canonicals  over 9 years ago

Commit id: b84b2378ed4b760366ba9c3b5b7d7bdc7362cf9a

deletions | additions      

         

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"level": 1,  "metadata": {},  "source": [  "Where to do  '3plus merged` overlap" ]  },  { 

],  "prompt_number": 17  },  {  "cell_type": "heading",  "level": 1,  "metadata": {},  "source": [  "Simpler Question - Gene Overlap?"  ]  },  {  "cell_type": "code",  "collapsed": false,  "input": [  "!ls analyses/dmr/"  ],  "language": "python",  "metadata": {},  "outputs": [  {  "output_type": "stream",  "stream": "stdout",  "text": [  "2M_3plusmerge_Hyper.gff 4M_3plusmerge_Hyper.gff 6M_3plusmerge_Hyper.gff\r\n",  "2M_3plusmerge_Hypo.gff 4M_3plusmerge_Hypo.gff 6M_3plusmerge_Hypo.gff\r\n"  ]  }  ],  "prompt_number": 20  },  {  "cell_type": "code",  "collapsed": false,  "input": [  "cd analyses/dmr/"  ],  "language": "python",  "metadata": {},  "outputs": [  {  "output_type": "stream",  "stream": "stdout",  "text": [  "/Users/sr320/git-repos/paper-Temp-stress/ipynb/analyses/dmr\n"  ]  }  ],  "prompt_number": 25  },  {  "cell_type": "code",  "collapsed": false,  "input": [  "%%bash\n",  "for f in *.gff\n",  "do\n",  "echo $f\n",  "intersectbed \\\n",  "-wb \\\n",  "-a $f \\\n",  "-b ../Cgigas_v9_gene.gff \\\n",  "> ../gene_intersect_\"$f\".txt\n",  "wc -l ../gene_intersect_\"$f\".txt\n",  "awk '{print $1$2$3}' ../gene_intersect_\"$f\".txt | sort | uniq | wc -l\n",  "done\n"  ],  "language": "python",  "metadata": {},  "outputs": [  {  "output_type": "stream",  "stream": "stdout",  "text": [  "2M_3plusmerge_Hyper.gff\n",  " 5 ../gene_intersect_2M_3plusmerge_Hyper.gff.txt\n",  " 4\n",  "2M_3plusmerge_Hypo.gff\n",  " 108 ../gene_intersect_2M_3plusmerge_Hypo.gff.txt\n",  " 106\n",  "4M_3plusmerge_Hyper.gff\n",  " 9 ../gene_intersect_4M_3plusmerge_Hyper.gff.txt\n",  " 9\n",  "4M_3plusmerge_Hypo.gff\n",  " 43 ../gene_intersect_4M_3plusmerge_Hypo.gff.txt\n",  " 42\n",  "6M_3plusmerge_Hyper.gff\n",  " 9 ../gene_intersect_6M_3plusmerge_Hyper.gff.txt\n",  " 9\n",  "6M_3plusmerge_Hypo.gff\n",  " 44 ../gene_intersect_6M_3plusmerge_Hypo.gff.txt\n",  " 42\n"  ]  }  ],  "prompt_number": 66  },  {  "cell_type": "code",  "collapsed": false,  "input": [  "!head ../gene_intersect_6M_3plusmerge_Hyper.gff.txt"  ],  "language": "python",  "metadata": {},  "outputs": [  {  "output_type": "stream",  "stream": "stdout",  "text": [  "scaffold1737\t485256\t485565\tscaffold1737\tGLEAN\tmRNA\t465811\t530668\t0.60401\t-\t.\tID=CGI_10018256;\r\n",  "scaffold1894\t222981\t223032\tscaffold1894\tGLEAN\tmRNA\t215043\t252780\t0.725039\t+\t.\tID=CGI_10013340;\r\n",  "scaffold1894\t223601\t223653\tscaffold1894\tGLEAN\tmRNA\t215043\t252780\t0.725039\t+\t.\tID=CGI_10013340;\r\n",  "scaffold1894\t224777\t224838\tscaffold1894\tGLEAN\tmRNA\t215043\t252780\t0.725039\t+\t.\tID=CGI_10013340;\r\n",  "scaffold392\t326519\t326818\tscaffold392\tGLEAN\tmRNA\t323342\t328165\t1\t+\t.\tID=CGI_10024212;\r\n",  "scaffold433\t204898\t205194\tscaffold433\tGLEAN\tmRNA\t203495\t226770\t0.837765\t+\t.\tID=CGI_10027060;\r\n",  "scaffold544\t44676\t44853\tscaffold544\tGLEAN\tmRNA\t43904\t52870\t0.999376\t-\t.\tID=CGI_10022545;\r\n",  "scaffold544\t45028\t45086\tscaffold544\tGLEAN\tmRNA\t43904\t52870\t0.999376\t-\t.\tID=CGI_10022545;\r\n",  "scaffold854\t353009\t353316\tscaffold854\tGLEAN\tmRNA\t352110\t370934\t0.574859\t+\t.\tID=CGI_10022251;\r\n"  ]  }  ],  "prompt_number": 67  },  {  "cell_type": "code",  "collapsed": false,  "input": [  "!cp 6M_3plusmerge_Hyper.gff 6M_3plusmerge_Hyper.bed"  ],  "language": "python",  "metadata": {},  "outputs": [],  "prompt_number": 69  },  {  "cell_type": "code",  "collapsed": false,  "input": [  "!head ./analyses/Cgigas_v9_gene.gff"  ],  "language": "python",  "metadata": {},  "outputs": [  {  "output_type": "stream",  "stream": "stdout",  "text": [  "C16582\tGLEAN\tmRNA\t35\t385\t0.555898\t-\t.\tID=CGI_10000001;\r\n",  "C17212\tGLEAN\tmRNA\t31\t363\t0.999572\t+\t.\tID=CGI_10000002;\r\n",  "C17316\tGLEAN\tmRNA\t30\t257\t0.555898\t+\t.\tID=CGI_10000003;\r\n",  "C17476\tGLEAN\tmRNA\t34\t257\t0.998947\t-\t.\tID=CGI_10000004;\r\n",  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"Creating Merged Features 3plus"  ]  },  {  "cell_type": "code",  "collapsed": false,  "input": [  "!wc -l ./data/2014.07.02.colson/genomeBrowserTracks/threeOrMoreAdjacentSigProbes/2014.07.02.2M_Hypo_3plusAdjactentProbes.gff"  ],  "language": "python",  "metadata": {},  "outputs": [  {  "output_type": "stream",  "stream": "stdout",  "text": [  " 310 ./data/2014.07.02.colson/genomeBrowserTracks/threeOrMoreAdjacentSigProbes/2014.07.02.2M_Hypo_3plusAdjactentProbes.gff\r\n"  ]  }  ],  "prompt_number": 14  },  {  "cell_type": "code",  "collapsed": false, 

"cell_type": "code",  "collapsed": false,  "input": [  "!cat ./analyses/[2-6]*.gff | cut -f1 | sort | uniq -c | sort" sort | tail"  ],  "language": "python",  "metadata": {}, 

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"outputs": [],  "prompt_number": 73  },  {  "cell_type": "heading",  "level": 1,  "metadata": {},  "source": [  "Product GTF of ONLY Diffexp genes"  ]  },  {  "cell_type": "code",  "collapsed": false,                                               

scaffold1583 976276 976596  scaffold257 1235165 1235481  scaffold43954 139271 139589  scaffold801 338541 338853           

scaffold1016 249436 249737  scaffold1041 199217 199498  scaffold1188 461425 461738  scaffold12 243960 244376  scaffold1210 165042 165474  scaffold1235 26466 26785  scaffold1255 198376 198802  scaffold1307 652152 652606  scaffold1322 409152 409472  scaffold1367 121175 121233  scaffold1367 121440 121625  scaffold1391 190523 190935  scaffold142 237803 238097  scaffold142 656269 656735  scaffold1437 20874 21168  scaffold1497 110217 110525  scaffold150 611097 611397  scaffold1503 67189 67517  scaffold1518 212930 213736  scaffold1532 1408496 1408798  scaffold1625 96879 97439  scaffold1627 214323 214619  scaffold163 482904 483345  scaffold1654 31753 32049  scaffold1656 72301 72470  scaffold1656 72788 72845  scaffold1737 503502 503553  scaffold1737 504057 504108  scaffold1737 504567 504618  scaffold1817 27146 27462  scaffold1835 389934 390487  scaffold189 209552 209991  scaffold1895 133360 133411  scaffold1895 133770 133821  scaffold1895 134200 134258  scaffold1895 134450 134505  scaffold198 550544 550846  scaffold200 34653 34969  scaffold200 35158 35314  scaffold22 1882179 1882231  scaffold22 1882425 1882588  scaffold226 264062 264360  scaffold241 85098 85539  scaffold242 76043 76344  scaffold247 141885 142442  scaffold247 144151 144583  scaffold313 960532 960833  scaffold394 61557 61874  scaffold394 555933 556224  scaffold39778 67963 68284  scaffold39778 68484 68538  scaffold4 76062 76429  scaffold40404 64481 64537  scaffold40404 64745 64917  scaffold40404 65770 65822  scaffold40404 66170 66221  scaffold40404 66370 66421  scaffold406 418360 418767  scaffold406 419005 419060  scaffold406 419330 419384  scaffold406 419660 419718  scaffold40832 34428 34730  scaffold41164 99229 99530  scaffold41900 43748 44054  scaffold42164 95203 95503  scaffold42380 90843 91129  scaffold43 407668 407845  scaffold43 408188 408243  scaffold43036 151198 151505  scaffold43074 200252 200583  scaffold43116 47628 48159  scaffold43258 205910 206238  scaffold44 372931 372983  scaffold44 373276 373703  scaffold44 373851 374293  scaffold44098 96033 96324  scaffold44098 195287 195573  scaffold481 576986 578532  scaffold492 344813 345249  scaffold521 66699 67000  scaffold541 376950 377606  scaffold544 49724 50385  scaffold58 142869 143053  scaffold58 143227 143289  scaffold588 244228 244534  scaffold601 1224731 1225027  scaffold614 224350 224641  scaffold616 239918 240109  scaffold616 240238 240404  scaffold616 240973 241033  scaffold616 282107 282402  scaffold63 419210 419515  scaffold632 252545 252986  scaffold709 412128 412179  scaffold709 412283 412869  scaffold713 340881 341162  scaffold737 107564 107860  scaffold802 110095 110279  scaffold802 110877 110939  scaffold847 550955 551123  scaffold847 551310 551368  scaffold853 46186 46496  scaffold879 121684 121740  scaffold879 121934 122121  scaffold885 474982 475403  scaffold894 42948 43267  scaffold983 16818 17119  scaffold983 17338 18139           

scaffold1241 28111 28419  scaffold1447 305772 306206  scaffold1805 257176 257496  scaffold257 1235165 1235481  scaffold40230 41604 41898  scaffold41982 74367 74671  scaffold63 426683 426993  scaffold70 774778 775094  scaffold801 338037 338345  scaffold801 339443 339729           

scaffold100 804406 804960  scaffold1018 367127 367319  scaffold1018 367618 367674  scaffold1077 401205 401534  scaffold12 244080 244376  scaffold1324 356489 356781  scaffold1409 139329 139505  scaffold1409 139629 139680  scaffold142 656144 656460  scaffold1484 346442 346623  scaffold1484 346732 346793  scaffold1518 212680 212981  scaffold1518 213195 213606  scaffold163 466915 466973  scaffold163 467170 467351  scaffold1759 85559 85855  scaffold1827 304885 305206  scaffold1834 57569 57746  scaffold1834 57946 58006  scaffold1901 351779 352066  scaffold219 171631 171798  scaffold219 171901 171954  scaffold242 75918 76344  scaffold247 141885 142442  scaffold274 459522 460223  scaffold388 212291 212349  scaffold388 213296 213462  scaffold40254 31331 31639  scaffold406 418610 418767  scaffold406 419005 419060  scaffold406 419330 419384  scaffold40856 88091 88283  scaffold40856 88593 88657  scaffold42326 136896 137187  scaffold42788 86761 86949  scaffold42788 87126 87188  scaffold481 577121 578407  scaffold531 87405 87712  scaffold542 250278 250740  scaffold557 401177 401740  scaffold57 375756 376179  scaffold594 259220 259403  scaffold594 259665 259723  scaffold747 349083 349421  scaffold853 46186 46496  scaffold880 495287 495584  scaffold968 118405 118571  scaffold968 118700 118751           

scaffold1737 485256 485565  scaffold1894 222981 223032  scaffold1894 223601 223653  scaffold1894 224777 224838  scaffold392 326519 326818  scaffold433 204898 205194  scaffold544 44676 44853  scaffold544 45028 45086  scaffold854 353009 353316           

scaffold1737 485256 485565  scaffold1894 222981 223032  scaffold1894 223601 223653  scaffold1894 224777 224838  scaffold392 326519 326818  scaffold433 204898 205194  scaffold544 44676 44853  scaffold544 45028 45086  scaffold854 353009 353316           

scaffold101 610174 610236  scaffold101 610559 610740  scaffold1123 40268 40589  scaffold1204 66531 66832  scaffold1235 34089 34268  scaffold1235 34383 34443  scaffold1249 494779 495082  scaffold1264 68697 69025  scaffold1493 16031 16478  scaffold1719 330025 330079  scaffold1719 330285 330484  scaffold1752 17551 17709  scaffold1752 17916 17975  scaffold193 65914 65965  scaffold193 66130 66185  scaffold193 66310 66371  scaffold203 259584 259908  scaffold247 141885 142442  scaffold247 144031 144583  scaffold308 50213 50645  scaffold335 60010 60067  scaffold335 60531 60693  scaffold348 716142 716446  scaffold372 176523 176578  scaffold372 176916 176971  scaffold372 177146 177197  scaffold392 203896 204208  scaffold392 213620 213932  scaffold394 555813 556110  scaffold40282 64161 64479  scaffold42302 144946 145003  scaffold42302 145180 145356  scaffold43794 261331 261398  scaffold43794 261706 261891  scaffold457 43443 43495  scaffold457 43703 43889  scaffold481 577121 577537  scaffold521 107296 107353  scaffold521 107545 107608  scaffold521 107905 107956  scaffold542 242268 242712  scaffold542 247837 248022  scaffold542 248198 248253  scaffold579 198644 198702  scaffold579 199044 199103  scaffold579 199314 199373  scaffold588 57559 57881  scaffold622 436892 437068  scaffold622 437182 437236  scaffold622 437447 437499  scaffold665 96792 96857  scaffold665 97417 97616  scaffold942 185812 186123           

scaffold1583 976276 976596 scaffold1583 GLEAN mRNA 953169 980453 0.999949 + . ID=CGI_10025857;  scaffold257 1235165 1235469 scaffold257 GLEAN mRNA 1234107 1235469 0.950391 + . ID=CGI_10027943;  scaffold43954 139271 139589 scaffold43954 GLEAN mRNA 139153 149832 0.999998 - . ID=CGI_10014576;  scaffold43954 139271 139589 scaffold43954 GLEAN mRNA 123161 140060 0.960284 + . ID=CGI_10014575;  scaffold801 338541 338853 scaffold801 GLEAN mRNA 337024 341103 0.651118 - . ID=CGI_10024086;           

scaffold1016 249436 249720 scaffold1016 GLEAN mRNA 243615 249720 0.734139 - . ID=CGI_10016890;  scaffold1041 199217 199498 scaffold1041 GLEAN mRNA 190887 201067 0.999676 + . ID=CGI_10014674;  scaffold1188 461425 461738 scaffold1188 GLEAN mRNA 459213 467928 1 - . ID=CGI_10025479;  scaffold12 243960 244376 scaffold12 GLEAN mRNA 239507 250665 0.994211 + . ID=CGI_10018033;  scaffold1210 165042 165448 scaffold1210 GLEAN mRNA 157915 165448 0.999351 - . ID=CGI_10014601;  scaffold1235 26466 26785 scaffold1235 GLEAN mRNA 16837 34725 0.738057 - . ID=CGI_10011828;  scaffold1255 198376 198802 scaffold1255 GLEAN mRNA 198297 207782 0.999944 + . ID=CGI_10018067;  scaffold1307 652152 652606 scaffold1307 GLEAN mRNA 646819 654061 0.999676 + . ID=CGI_10023776;  scaffold1322 409152 409376 scaffold1322 GLEAN mRNA 394198 409376 0.999229 - . ID=CGI_10019477;  scaffold1367 121175 121233 scaffold1367 GLEAN mRNA 110543 127160 0.879341 - . ID=CGI_10005500;  scaffold1367 121440 121625 scaffold1367 GLEAN mRNA 110543 127160 0.879341 - . ID=CGI_10005500;  scaffold142 237803 238097 scaffold142 GLEAN mRNA 226750 254431 0.969071 + . ID=CGI_10026760;  scaffold142 656269 656735 scaffold142 GLEAN mRNA 652587 657482 0.927019 + . ID=CGI_10026768;  scaffold1437 20874 21168 scaffold1437 GLEAN mRNA 9816 21221 0.99909 + . ID=CGI_10003664;  scaffold1497 110217 110525 scaffold1497 GLEAN mRNA 95806 128213 0.600834 - . ID=CGI_10006965;  scaffold150 611097 611397 scaffold150 GLEAN mRNA 591454 628379 0.472758 + . ID=CGI_10028616;  scaffold1503 67189 67517 scaffold1503 GLEAN mRNA 55967 67698 1 + . ID=CGI_10009528;  scaffold1518 213310 213540 scaffold1518 GLEAN mRNA 213310 213540 0.575104 - . ID=CGI_10017802;  scaffold1518 212930 213736 scaffold1518 GLEAN mRNA 210347 219718 0.999512 + . ID=CGI_10017800;  scaffold1518 212930 213237 scaffold1518 GLEAN mRNA 212842 213237 0.999996 - . ID=CGI_10017801;  scaffold1532 1408496 1408798 scaffold1532 GLEAN mRNA 1408196 1409146 0.999661 - . ID=CGI_10028292;  scaffold1625 96879 97439 scaffold1625 GLEAN mRNA 90845 97594 0.999364 + . ID=CGI_10007529;  scaffold1627 214323 214619 scaffold1627 GLEAN mRNA 173064 218682 0.503235 - . ID=CGI_10009567;  scaffold163 482904 483345 scaffold163 GLEAN mRNA 440738 483510 0.311673 + . ID=CGI_10018629;  scaffold1654 31753 32049 scaffold1654 GLEAN mRNA 27749 35815 0.503036 + . ID=CGI_10004123;  scaffold1656 72301 72470 scaffold1656 GLEAN mRNA 45907 73911 0.308617 - . ID=CGI_10006142;  scaffold1656 72788 72845 scaffold1656 GLEAN mRNA 45907 73911 0.308617 - . ID=CGI_10006142;  scaffold1737 503502 503553 scaffold1737 GLEAN mRNA 465811 530668 0.60401 - . ID=CGI_10018256;  scaffold1737 504057 504108 scaffold1737 GLEAN mRNA 465811 530668 0.60401 - . ID=CGI_10018256;  scaffold1737 504567 504618 scaffold1737 GLEAN mRNA 465811 530668 0.60401 - . ID=CGI_10018256;  scaffold1817 27146 27462 scaffold1817 GLEAN mRNA 9897 44966 0.453567 - . ID=CGI_10005250;  scaffold1835 389934 390487 scaffold1835 GLEAN mRNA 385259 393032 1 - . ID=CGI_10015195;  scaffold189 209552 209991 scaffold189 GLEAN mRNA 162558 220166 0.999165 - . ID=CGI_10018675;  scaffold1895 133360 133411 scaffold1895 GLEAN mRNA 126234 162235 0.811929 - . ID=CGI_10010979;  scaffold1895 133770 133821 scaffold1895 GLEAN mRNA 126234 162235 0.811929 - . ID=CGI_10010979;  scaffold1895 134200 134258 scaffold1895 GLEAN mRNA 126234 162235 0.811929 - . ID=CGI_10010979;  scaffold1895 134450 134505 scaffold1895 GLEAN mRNA 126234 162235 0.811929 - . ID=CGI_10010979;  scaffold198 550544 550846 scaffold198 GLEAN mRNA 532542 554203 0.338011 + . ID=CGI_10027815;  scaffold200 34653 34969 scaffold200 GLEAN mRNA 28354 40232 0.644312 + . ID=CGI_10006223;  scaffold200 35158 35314 scaffold200 GLEAN mRNA 28354 40232 0.644312 + . ID=CGI_10006223;  scaffold22 1882179 1882231 scaffold22 GLEAN mRNA 1869336 1885890 0.999933 - . ID=CGI_10028939;  scaffold22 1882425 1882588 scaffold22 GLEAN mRNA 1869336 1885890 0.999933 - . ID=CGI_10028939;  scaffold226 264062 264360 scaffold226 GLEAN mRNA 263267 278066 0.893615 + . ID=CGI_10025348;  scaffold241 85098 85539 scaffold241 GLEAN mRNA 81964 102317 0.534439 + . ID=CGI_10025091;  scaffold242 76043 76344 scaffold242 GLEAN mRNA 75832 84666 0.771995 + . ID=CGI_10007083;  scaffold247 141885 142442 scaffold247 GLEAN mRNA 139499 148449 0.991247 + . ID=CGI_10018592;  scaffold247 144151 144583 scaffold247 GLEAN mRNA 139499 148449 0.991247 + . ID=CGI_10018592;  scaffold313 960532 960833 scaffold313 GLEAN mRNA 960141 974099 0.950119 + . ID=CGI_10026629;  scaffold394 61557 61874 scaffold394 GLEAN mRNA 55517 68616 0.840059 + . ID=CGI_10022063;  scaffold394 555933 556224 scaffold394 GLEAN mRNA 549658 560561 0.811205 + . ID=CGI_10022093;  scaffold39778 67963 68284 scaffold39778 GLEAN mRNA 56712 68685 0.806384 - . ID=CGI_10003612;  scaffold39778 68484 68538 scaffold39778 GLEAN mRNA 56712 68685 0.806384 - . ID=CGI_10003612;  scaffold4 76062 76429 scaffold4 GLEAN mRNA 65110 80628 0.813891 + . ID=CGI_10021163;  scaffold40404 64481 64537 scaffold40404 GLEAN mRNA 60176 84997 0.660218 - . ID=CGI_10004086;  scaffold40404 64745 64917 scaffold40404 GLEAN mRNA 60176 84997 0.660218 - . ID=CGI_10004086;  scaffold40404 65770 65822 scaffold40404 GLEAN mRNA 60176 84997 0.660218 - . ID=CGI_10004086;  scaffold40404 66170 66221 scaffold40404 GLEAN mRNA 60176 84997 0.660218 - . ID=CGI_10004086;  scaffold40404 66370 66421 scaffold40404 GLEAN mRNA 60176 84997 0.660218 - . ID=CGI_10004086;  scaffold406 418360 418767 scaffold406 GLEAN mRNA 402781 436007 0.999901 + . ID=CGI_10023847;  scaffold406 419005 419060 scaffold406 GLEAN mRNA 402781 436007 0.999901 + . ID=CGI_10023847;  scaffold406 419330 419384 scaffold406 GLEAN mRNA 402781 436007 0.999901 + . ID=CGI_10023847;  scaffold406 419660 419718 scaffold406 GLEAN mRNA 402781 436007 0.999901 + . ID=CGI_10023847;  scaffold40832 34428 34730 scaffold40832 GLEAN mRNA 32030 40719 0.998954 + . ID=CGI_10004454;  scaffold41164 99229 99530 scaffold41164 GLEAN mRNA 83753 104051 0.95986 - . ID=CGI_10004764;  scaffold41900 43748 44054 scaffold41900 GLEAN mRNA 42792 46989 0.211753 - . ID=CGI_10005816;  scaffold42164 95203 95503 scaffold42164 GLEAN mRNA 89959 98423 0.999572 + . ID=CGI_10006321;  scaffold42380 90843 91129 scaffold42380 GLEAN mRNA 82419 110422 1 + . ID=CGI_10006731;  scaffold43 407668 407845 scaffold43 GLEAN mRNA 380222 414471 0.256408 - . ID=CGI_10021355;  scaffold43 408188 408243 scaffold43 GLEAN mRNA 380222 414471 0.256408 - . ID=CGI_10021355;  scaffold43036 151198 151505 scaffold43036 GLEAN mRNA 148188 171820 0.999804 + . ID=CGI_10008498;  scaffold43074 200252 200583 scaffold43074 GLEAN mRNA 176086 213395 0.431597 + . ID=CGI_10008693;  scaffold43116 47628 48159 scaffold43116 GLEAN mRNA 47223 64768 0.988376 + . ID=CGI_10008808;  scaffold43258 205910 206238 scaffold43258 GLEAN mRNA 199849 232336 0.614349 + . ID=CGI_10009635;  scaffold44 372931 372983 scaffold44 GLEAN mRNA 369681 374648 1 - . ID=CGI_10016838;  scaffold44 373276 373703 scaffold44 GLEAN mRNA 369681 374648 1 - . ID=CGI_10016838;  scaffold44 373851 374293 scaffold44 GLEAN mRNA 369681 374648 1 - . ID=CGI_10016838;  scaffold44098 96033 96324 scaffold44098 GLEAN mRNA 95889 97046 0.999661 + . ID=CGI_10017701;  scaffold44098 195287 195573 scaffold44098 GLEAN mRNA 188555 213785 0.544557 + . ID=CGI_10017708;  scaffold481 576986 578532 scaffold481 GLEAN mRNA 564527 580694 0.999885 - . ID=CGI_10027225;  scaffold492 344813 345249 scaffold492 GLEAN mRNA 344244 345458 1 + . ID=CGI_10024825;  scaffold521 66699 67000 scaffold521 GLEAN mRNA 38898 72859 0.840523 - . ID=CGI_10009384;  scaffold541 376950 377606 scaffold541 GLEAN mRNA 373407 387637 0.761514 - . ID=CGI_10022391;  scaffold544 49724 50385 scaffold544 GLEAN mRNA 43904 52870 0.999376 - . ID=CGI_10022545;  scaffold58 142869 143053 scaffold58 GLEAN mRNA 136138 172120 0.0334817 + . ID=CGI_10023136;  scaffold58 143227 143289 scaffold58 GLEAN mRNA 136138 172120 0.0334817 + . ID=CGI_10023136;  scaffold588 244228 244534 scaffold588 GLEAN mRNA 236852 247074 0.99937 - . ID=CGI_10016217;  scaffold601 1224731 1225027 scaffold601 GLEAN mRNA 1201024 1226827 0.805376 - . ID=CGI_10026861;  scaffold614 224350 224641 scaffold614 GLEAN mRNA 191646 225639 0.772957 + . ID=CGI_10016263;  scaffold616 239918 240109 scaffold616 GLEAN mRNA 235595 244727 0.999676 - . ID=CGI_10015397;  scaffold616 240238 240404 scaffold616 GLEAN mRNA 235595 244727 0.999676 - . ID=CGI_10015397;  scaffold616 240973 241033 scaffold616 GLEAN mRNA 235595 244727 0.999676 - . ID=CGI_10015397;  scaffold616 282107 282402 scaffold616 GLEAN mRNA 278468 304529 0.291668 - . ID=CGI_10015401;  scaffold63 419210 419515 scaffold63 GLEAN mRNA 406163 425324 1 - . ID=CGI_10017281;  scaffold632 252545 252986 scaffold632 GLEAN mRNA 251262 255233 0.888794 + . ID=CGI_10018448;  scaffold709 412128 412179 scaffold709 GLEAN mRNA 411384 420120 0.999868 - . ID=CGI_10020093;  scaffold709 412128 412179 scaffold709 GLEAN mRNA 407943 427071 0.574856 + . ID=CGI_10020092;  scaffold709 412283 412869 scaffold709 GLEAN mRNA 411384 420120 0.999868 - . ID=CGI_10020093;  scaffold709 412283 412869 scaffold709 GLEAN mRNA 407943 427071 0.574856 + . ID=CGI_10020092;  scaffold713 340881 341162 scaffold713 GLEAN mRNA 338450 341603 1 + . ID=CGI_10017753;  scaffold737 107564 107860 scaffold737 GLEAN mRNA 107414 109861 0.999015 + . ID=CGI_10014308;  scaffold802 110095 110279 scaffold802 GLEAN mRNA 107008 130874 0.996366 + . ID=CGI_10015726;  scaffold802 110877 110939 scaffold802 GLEAN mRNA 107008 130874 0.996366 + . ID=CGI_10015726;  scaffold879 121684 121740 scaffold879 GLEAN mRNA 83884 129121 0.946613 - . ID=CGI_10007573;  scaffold879 121934 122121 scaffold879 GLEAN mRNA 83884 129121 0.946613 - . ID=CGI_10007573;  scaffold885 474982 475403 scaffold885 GLEAN mRNA 474594 488964 0.69287 - . ID=CGI_10025208;  scaffold894 42948 43267 scaffold894 GLEAN mRNA 42935 58269 0.999999 + . ID=CGI_10008538;  scaffold983 16962 17119 scaffold983 GLEAN mRNA 16962 18259 0.550884 + . ID=CGI_10014023;  scaffold983 17338 18139 scaffold983 GLEAN mRNA 16962 18259 0.550884 + . ID=CGI_10014023;           

scaffold1016 249436 249720 scaffold1016 GLEAN mRNA 243615 249720 0.734139 - . ID=CGI_10016890;  scaffold1041 199217 199498 scaffold1041 GLEAN mRNA 190887 201067 0.999676 + . ID=CGI_10014674;  scaffold1188 461425 461738 scaffold1188 GLEAN mRNA 459213 467928 1 - . ID=CGI_10025479;  scaffold12 243960 244376 scaffold12 GLEAN mRNA 239507 250665 0.994211 + . ID=CGI_10018033;  scaffold1210 165042 165448 scaffold1210 GLEAN mRNA 157915 165448 0.999351 - . ID=CGI_10014601;  scaffold1235 26466 26785 scaffold1235 GLEAN mRNA 16837 34725 0.738057 - . ID=CGI_10011828;  scaffold1255 198376 198802 scaffold1255 GLEAN mRNA 198297 207782 0.999944 + . ID=CGI_10018067;  scaffold1307 652152 652606 scaffold1307 GLEAN mRNA 646819 654061 0.999676 + . ID=CGI_10023776;  scaffold1322 409152 409376 scaffold1322 GLEAN mRNA 394198 409376 0.999229 - . ID=CGI_10019477;  scaffold1367 121175 121233 scaffold1367 GLEAN mRNA 110543 127160 0.879341 - . ID=CGI_10005500;  scaffold1367 121440 121625 scaffold1367 GLEAN mRNA 110543 127160 0.879341 - . ID=CGI_10005500;  scaffold142 237803 238097 scaffold142 GLEAN mRNA 226750 254431 0.969071 + . ID=CGI_10026760;  scaffold142 656269 656735 scaffold142 GLEAN mRNA 652587 657482 0.927019 + . ID=CGI_10026768;  scaffold1437 20874 21168 scaffold1437 GLEAN mRNA 9816 21221 0.99909 + . ID=CGI_10003664;  scaffold1497 110217 110525 scaffold1497 GLEAN mRNA 95806 128213 0.600834 - . ID=CGI_10006965;  scaffold150 611097 611397 scaffold150 GLEAN mRNA 591454 628379 0.472758 + . ID=CGI_10028616;  scaffold1503 67189 67517 scaffold1503 GLEAN mRNA 55967 67698 1 + . ID=CGI_10009528;  scaffold1518 213310 213540 scaffold1518 GLEAN mRNA 213310 213540 0.575104 - . ID=CGI_10017802;  scaffold1518 212930 213736 scaffold1518 GLEAN mRNA 210347 219718 0.999512 + . ID=CGI_10017800;  scaffold1518 212930 213237 scaffold1518 GLEAN mRNA 212842 213237 0.999996 - . ID=CGI_10017801;  scaffold1532 1408496 1408798 scaffold1532 GLEAN mRNA 1408196 1409146 0.999661 - . ID=CGI_10028292;  scaffold1625 96879 97439 scaffold1625 GLEAN mRNA 90845 97594 0.999364 + . ID=CGI_10007529;  scaffold1627 214323 214619 scaffold1627 GLEAN mRNA 173064 218682 0.503235 - . ID=CGI_10009567;  scaffold163 482904 483345 scaffold163 GLEAN mRNA 440738 483510 0.311673 + . ID=CGI_10018629;  scaffold1654 31753 32049 scaffold1654 GLEAN mRNA 27749 35815 0.503036 + . ID=CGI_10004123;  scaffold1656 72301 72470 scaffold1656 GLEAN mRNA 45907 73911 0.308617 - . ID=CGI_10006142;  scaffold1656 72788 72845 scaffold1656 GLEAN mRNA 45907 73911 0.308617 - . ID=CGI_10006142;  scaffold1737 503502 503553 scaffold1737 GLEAN mRNA 465811 530668 0.60401 - . ID=CGI_10018256;  scaffold1737 504057 504108 scaffold1737 GLEAN mRNA 465811 530668 0.60401 - . ID=CGI_10018256;  scaffold1737 504567 504618 scaffold1737 GLEAN mRNA 465811 530668 0.60401 - . ID=CGI_10018256;  scaffold1817 27146 27462 scaffold1817 GLEAN mRNA 9897 44966 0.453567 - . ID=CGI_10005250;  scaffold1835 389934 390487 scaffold1835 GLEAN mRNA 385259 393032 1 - . ID=CGI_10015195;  scaffold189 209552 209991 scaffold189 GLEAN mRNA 162558 220166 0.999165 - . ID=CGI_10018675;  scaffold1895 133360 133411 scaffold1895 GLEAN mRNA 126234 162235 0.811929 - . ID=CGI_10010979;  scaffold1895 133770 133821 scaffold1895 GLEAN mRNA 126234 162235 0.811929 - . ID=CGI_10010979;  scaffold1895 134200 134258 scaffold1895 GLEAN mRNA 126234 162235 0.811929 - . ID=CGI_10010979;  scaffold1895 134450 134505 scaffold1895 GLEAN mRNA 126234 162235 0.811929 - . ID=CGI_10010979;  scaffold198 550544 550846 scaffold198 GLEAN mRNA 532542 554203 0.338011 + . ID=CGI_10027815;  scaffold200 34653 34969 scaffold200 GLEAN mRNA 28354 40232 0.644312 + . ID=CGI_10006223;  scaffold200 35158 35314 scaffold200 GLEAN mRNA 28354 40232 0.644312 + . ID=CGI_10006223;  scaffold22 1882179 1882231 scaffold22 GLEAN mRNA 1869336 1885890 0.999933 - . ID=CGI_10028939;  scaffold22 1882425 1882588 scaffold22 GLEAN mRNA 1869336 1885890 0.999933 - . ID=CGI_10028939;  scaffold226 264062 264360 scaffold226 GLEAN mRNA 263267 278066 0.893615 + . ID=CGI_10025348;  scaffold241 85098 85539 scaffold241 GLEAN mRNA 81964 102317 0.534439 + . ID=CGI_10025091;  scaffold242 76043 76344 scaffold242 GLEAN mRNA 75832 84666 0.771995 + . ID=CGI_10007083;  scaffold247 141885 142442 scaffold247 GLEAN mRNA 139499 148449 0.991247 + . ID=CGI_10018592;  scaffold247 144151 144583 scaffold247 GLEAN mRNA 139499 148449 0.991247 + . ID=CGI_10018592;  scaffold313 960532 960833 scaffold313 GLEAN mRNA 960141 974099 0.950119 + . ID=CGI_10026629;  scaffold394 61557 61874 scaffold394 GLEAN mRNA 55517 68616 0.840059 + . ID=CGI_10022063;  scaffold394 555933 556224 scaffold394 GLEAN mRNA 549658 560561 0.811205 + . ID=CGI_10022093;  scaffold39778 67963 68284 scaffold39778 GLEAN mRNA 56712 68685 0.806384 - . ID=CGI_10003612;  scaffold39778 68484 68538 scaffold39778 GLEAN mRNA 56712 68685 0.806384 - . ID=CGI_10003612;  scaffold4 76062 76429 scaffold4 GLEAN mRNA 65110 80628 0.813891 + . ID=CGI_10021163;  scaffold40404 64481 64537 scaffold40404 GLEAN mRNA 60176 84997 0.660218 - . ID=CGI_10004086;  scaffold40404 64745 64917 scaffold40404 GLEAN mRNA 60176 84997 0.660218 - . ID=CGI_10004086;  scaffold40404 65770 65822 scaffold40404 GLEAN mRNA 60176 84997 0.660218 - . ID=CGI_10004086;  scaffold40404 66170 66221 scaffold40404 GLEAN mRNA 60176 84997 0.660218 - . ID=CGI_10004086;  scaffold40404 66370 66421 scaffold40404 GLEAN mRNA 60176 84997 0.660218 - . ID=CGI_10004086;  scaffold406 418360 418767 scaffold406 GLEAN mRNA 402781 436007 0.999901 + . ID=CGI_10023847;  scaffold406 419005 419060 scaffold406 GLEAN mRNA 402781 436007 0.999901 + . ID=CGI_10023847;  scaffold406 419330 419384 scaffold406 GLEAN mRNA 402781 436007 0.999901 + . ID=CGI_10023847;  scaffold406 419660 419718 scaffold406 GLEAN mRNA 402781 436007 0.999901 + . ID=CGI_10023847;  scaffold40832 34428 34730 scaffold40832 GLEAN mRNA 32030 40719 0.998954 + . ID=CGI_10004454;  scaffold41164 99229 99530 scaffold41164 GLEAN mRNA 83753 104051 0.95986 - . ID=CGI_10004764;  scaffold41900 43748 44054 scaffold41900 GLEAN mRNA 42792 46989 0.211753 - . ID=CGI_10005816;  scaffold42164 95203 95503 scaffold42164 GLEAN mRNA 89959 98423 0.999572 + . ID=CGI_10006321;  scaffold42380 90843 91129 scaffold42380 GLEAN mRNA 82419 110422 1 + . ID=CGI_10006731;  scaffold43 407668 407845 scaffold43 GLEAN mRNA 380222 414471 0.256408 - . ID=CGI_10021355;  scaffold43 408188 408243 scaffold43 GLEAN mRNA 380222 414471 0.256408 - . ID=CGI_10021355;  scaffold43036 151198 151505 scaffold43036 GLEAN mRNA 148188 171820 0.999804 + . ID=CGI_10008498;  scaffold43074 200252 200583 scaffold43074 GLEAN mRNA 176086 213395 0.431597 + . ID=CGI_10008693;  scaffold43116 47628 48159 scaffold43116 GLEAN mRNA 47223 64768 0.988376 + . ID=CGI_10008808;  scaffold43258 205910 206238 scaffold43258 GLEAN mRNA 199849 232336 0.614349 + . ID=CGI_10009635;  scaffold44 372931 372983 scaffold44 GLEAN mRNA 369681 374648 1 - . ID=CGI_10016838;  scaffold44 373276 373703 scaffold44 GLEAN mRNA 369681 374648 1 - . ID=CGI_10016838;  scaffold44 373851 374293 scaffold44 GLEAN mRNA 369681 374648 1 - . ID=CGI_10016838;  scaffold44098 96033 96324 scaffold44098 GLEAN mRNA 95889 97046 0.999661 + . ID=CGI_10017701;  scaffold44098 195287 195573 scaffold44098 GLEAN mRNA 188555 213785 0.544557 + . ID=CGI_10017708;  scaffold481 576986 578532 scaffold481 GLEAN mRNA 564527 580694 0.999885 - . ID=CGI_10027225;  scaffold492 344813 345249 scaffold492 GLEAN mRNA 344244 345458 1 + . ID=CGI_10024825;  scaffold521 66699 67000 scaffold521 GLEAN mRNA 38898 72859 0.840523 - . ID=CGI_10009384;  scaffold541 376950 377606 scaffold541 GLEAN mRNA 373407 387637 0.761514 - . ID=CGI_10022391;  scaffold544 49724 50385 scaffold544 GLEAN mRNA 43904 52870 0.999376 - . ID=CGI_10022545;  scaffold58 142869 143053 scaffold58 GLEAN mRNA 136138 172120 0.0334817 + . ID=CGI_10023136;  scaffold58 143227 143289 scaffold58 GLEAN mRNA 136138 172120 0.0334817 + . ID=CGI_10023136;  scaffold588 244228 244534 scaffold588 GLEAN mRNA 236852 247074 0.99937 - . ID=CGI_10016217;  scaffold601 1224731 1225027 scaffold601 GLEAN mRNA 1201024 1226827 0.805376 - . ID=CGI_10026861;  scaffold614 224350 224641 scaffold614 GLEAN mRNA 191646 225639 0.772957 + . ID=CGI_10016263;  scaffold616 239918 240109 scaffold616 GLEAN mRNA 235595 244727 0.999676 - . ID=CGI_10015397;  scaffold616 240238 240404 scaffold616 GLEAN mRNA 235595 244727 0.999676 - . ID=CGI_10015397;  scaffold616 240973 241033 scaffold616 GLEAN mRNA 235595 244727 0.999676 - . ID=CGI_10015397;  scaffold616 282107 282402 scaffold616 GLEAN mRNA 278468 304529 0.291668 - . ID=CGI_10015401;  scaffold63 419210 419515 scaffold63 GLEAN mRNA 406163 425324 1 - . ID=CGI_10017281;  scaffold632 252545 252986 scaffold632 GLEAN mRNA 251262 255233 0.888794 + . ID=CGI_10018448;  scaffold709 412128 412179 scaffold709 GLEAN mRNA 411384 420120 0.999868 - . ID=CGI_10020093;  scaffold709 412128 412179 scaffold709 GLEAN mRNA 407943 427071 0.574856 + . ID=CGI_10020092;  scaffold709 412283 412869 scaffold709 GLEAN mRNA 411384 420120 0.999868 - . ID=CGI_10020093;  scaffold709 412283 412869 scaffold709 GLEAN mRNA 407943 427071 0.574856 + . ID=CGI_10020092;  scaffold713 340881 341162 scaffold713 GLEAN mRNA 338450 341603 1 + . ID=CGI_10017753;  scaffold737 107564 107860 scaffold737 GLEAN mRNA 107414 109861 0.999015 + . ID=CGI_10014308;  scaffold802 110095 110279 scaffold802 GLEAN mRNA 107008 130874 0.996366 + . ID=CGI_10015726;  scaffold802 110877 110939 scaffold802 GLEAN mRNA 107008 130874 0.996366 + . ID=CGI_10015726;  scaffold879 121684 121740 scaffold879 GLEAN mRNA 83884 129121 0.946613 - . ID=CGI_10007573;  scaffold879 121934 122121 scaffold879 GLEAN mRNA 83884 129121 0.946613 - . ID=CGI_10007573;  scaffold885 474982 475403 scaffold885 GLEAN mRNA 474594 488964 0.69287 - . ID=CGI_10025208;  scaffold894 42948 43267 scaffold894 GLEAN mRNA 42935 58269 0.999999 + . ID=CGI_10008538;  scaffold983 16962 17119 scaffold983 GLEAN mRNA 16962 18259 0.550884 + . ID=CGI_10014023;  scaffold983 17338 18139 scaffold983 GLEAN mRNA 16962 18259 0.550884 + . ID=CGI_10014023;           

scaffold1241 28111 28363 scaffold1241 GLEAN mRNA 21350 28363 0.999676 + . ID=CGI_10016417;  scaffold1447 305772 306206 scaffold1447 GLEAN mRNA 300093 324572 0.937847 - . ID=CGI_10013830;  scaffold1805 257176 257496 scaffold1805 GLEAN mRNA 255030 268289 0.472749 - . ID=CGI_10012447;  scaffold257 1235165 1235469 scaffold257 GLEAN mRNA 1234107 1235469 0.950391 + . ID=CGI_10027943;  scaffold40230 41604 41898 scaffold40230 GLEAN mRNA 15231 44144 0.998984 + . ID=CGI_10003959;  scaffold41982 74367 74671 scaffold41982 GLEAN mRNA 68195 75399 0.820931 - . ID=CGI_10005972;  scaffold70 774778 775094 scaffold70 GLEAN mRNA 764546 796463 0.934901 - . ID=CGI_10024790;  scaffold801 338037 338345 scaffold801 GLEAN mRNA 337024 341103 0.651118 - . ID=CGI_10024086;  scaffold801 339443 339729 scaffold801 GLEAN mRNA 337024 341103 0.651118 - . ID=CGI_10024086;           

scaffold100 804406 804960 scaffold100 GLEAN mRNA 803879 806686 0.588753 + . ID=CGI_10026457;  scaffold1077 401205 401534 scaffold1077 GLEAN mRNA 387371 414102 0.296665 + . ID=CGI_10017830;  scaffold12 244080 244376 scaffold12 GLEAN mRNA 239507 250665 0.994211 + . ID=CGI_10018033;  scaffold1324 356489 356781 scaffold1324 GLEAN mRNA 354089 362762 1 + . ID=CGI_10018396;  scaffold1409 139329 139505 scaffold1409 GLEAN mRNA 123560 153776 0.210676 - . ID=CGI_10013783;  scaffold1409 139629 139680 scaffold1409 GLEAN mRNA 123560 153776 0.210676 - . ID=CGI_10013783;  scaffold142 656144 656460 scaffold142 GLEAN mRNA 652587 657482 0.927019 + . ID=CGI_10026768;  scaffold1484 346442 346623 scaffold1484 GLEAN mRNA 342488 349065 0.84036 - . ID=CGI_10013272;  scaffold1484 346732 346793 scaffold1484 GLEAN mRNA 342488 349065 0.84036 - . ID=CGI_10013272;  scaffold1518 212680 212981 scaffold1518 GLEAN mRNA 210347 219718 0.999512 + . ID=CGI_10017800;  scaffold1518 212842 212981 scaffold1518 GLEAN mRNA 212842 213237 0.999996 - . ID=CGI_10017801;  scaffold1518 213310 213540 scaffold1518 GLEAN mRNA 213310 213540 0.575104 - . ID=CGI_10017802;  scaffold1518 213195 213606 scaffold1518 GLEAN mRNA 210347 219718 0.999512 + . ID=CGI_10017800;  scaffold1518 213195 213237 scaffold1518 GLEAN mRNA 212842 213237 0.999996 - . ID=CGI_10017801;  scaffold163 466915 466973 scaffold163 GLEAN mRNA 440738 483510 0.311673 + . ID=CGI_10018629;  scaffold163 467170 467351 scaffold163 GLEAN mRNA 440738 483510 0.311673 + . ID=CGI_10018629;  scaffold1759 85559 85855 scaffold1759 GLEAN mRNA 83905 106418 0.889634 + . ID=CGI_10010906;  scaffold1827 304885 305206 scaffold1827 GLEAN mRNA 300627 306901 0.449006 + . ID=CGI_10013808;  scaffold1834 57569 57746 scaffold1834 GLEAN mRNA 40709 65850 0.846288 + . ID=CGI_10016651;  scaffold1834 57946 58006 scaffold1834 GLEAN mRNA 40709 65850 0.846288 + . ID=CGI_10016651;  scaffold242 75918 76344 scaffold242 GLEAN mRNA 75832 84666 0.771995 + . ID=CGI_10007083;  scaffold247 141885 142442 scaffold247 GLEAN mRNA 139499 148449 0.991247 + . ID=CGI_10018592;  scaffold274 459522 460223 scaffold274 GLEAN mRNA 442893 461703 0.999674 - . ID=CGI_10017036;  scaffold388 212291 212349 scaffold388 GLEAN mRNA 211826 226750 0.996237 + . ID=CGI_10020465;  scaffold388 213296 213462 scaffold388 GLEAN mRNA 211826 226750 0.996237 + . ID=CGI_10020465;  scaffold40254 31331 31639 scaffold40254 GLEAN mRNA 31193 43032 1 + . ID=CGI_10004006;  scaffold406 418610 418767 scaffold406 GLEAN mRNA 402781 436007 0.999901 + . ID=CGI_10023847;  scaffold406 419005 419060 scaffold406 GLEAN mRNA 402781 436007 0.999901 + . ID=CGI_10023847;  scaffold406 419330 419384 scaffold406 GLEAN mRNA 402781 436007 0.999901 + . ID=CGI_10023847;  scaffold40856 88091 88283 scaffold40856 GLEAN mRNA 85567 90161 0.999595 - . ID=CGI_10004527;  scaffold40856 88593 88657 scaffold40856 GLEAN mRNA 85567 90161 0.999595 - . ID=CGI_10004527;  scaffold42326 136896 137187 scaffold42326 GLEAN mRNA 118399 154714 0.498922 - . ID=CGI_10006698;  scaffold42788 86761 86949 scaffold42788 GLEAN mRNA 24369 91602 0.998319 - . ID=CGI_10007754;  scaffold42788 87126 87188 scaffold42788 GLEAN mRNA 24369 91602 0.998319 - . ID=CGI_10007754;  scaffold481 577121 578407 scaffold481 GLEAN mRNA 564527 580694 0.999885 - . ID=CGI_10027225;  scaffold531 87405 87712 scaffold531 GLEAN mRNA 81235 99394 0.999624 - . ID=CGI_10014490;  scaffold542 250278 250299 scaffold542 GLEAN mRNA 230390 250299 0.926479 - . ID=CGI_10014766;  scaffold557 401528 401740 scaffold557 GLEAN mRNA 401528 413927 0.99928 + . ID=CGI_10017980;  scaffold57 375756 376179 scaffold57 GLEAN mRNA 361110 382565 0.943475 + . ID=CGI_10020552;  scaffold57 375756 376179 scaffold57 GLEAN mRNA 361168 396087 0.322076 - . ID=CGI_10020553;  scaffold880 495287 495584 scaffold880 GLEAN mRNA 490990 515117 0.998312 - . ID=CGI_10022196;  scaffold968 118405 118571 scaffold968 GLEAN mRNA 110087 120737 0.425641 + . ID=CGI_10006297;  scaffold968 118700 118751 scaffold968 GLEAN mRNA 110087 120737 0.425641 + . ID=CGI_10006297;           

scaffold1737 485256 485565 scaffold1737 GLEAN mRNA 465811 530668 0.60401 - . ID=CGI_10018256;  scaffold1894 222981 223032 scaffold1894 GLEAN mRNA 215043 252780 0.725039 + . ID=CGI_10013340;  scaffold1894 223601 223653 scaffold1894 GLEAN mRNA 215043 252780 0.725039 + . ID=CGI_10013340;  scaffold1894 224777 224838 scaffold1894 GLEAN mRNA 215043 252780 0.725039 + . ID=CGI_10013340;  scaffold392 326519 326818 scaffold392 GLEAN mRNA 323342 328165 1 + . ID=CGI_10024212;  scaffold433 204898 205194 scaffold433 GLEAN mRNA 203495 226770 0.837765 + . ID=CGI_10027060;  scaffold544 44676 44853 scaffold544 GLEAN mRNA 43904 52870 0.999376 - . ID=CGI_10022545;  scaffold544 45028 45086 scaffold544 GLEAN mRNA 43904 52870 0.999376 - . ID=CGI_10022545;  scaffold854 353009 353316 scaffold854 GLEAN mRNA 352110 370934 0.574859 + . ID=CGI_10022251;           

scaffold101 610174 610236 scaffold101 GLEAN mRNA 603146 610994 0.999615 - . ID=CGI_10021652;  scaffold101 610559 610740 scaffold101 GLEAN mRNA 603146 610994 0.999615 - . ID=CGI_10021652;  scaffold1204 66531 66832 scaffold1204 GLEAN mRNA 59148 67801 0.377863 - . ID=CGI_10009807;  scaffold1235 34089 34268 scaffold1235 GLEAN mRNA 16837 34725 0.738057 - . ID=CGI_10011828;  scaffold1235 34383 34443 scaffold1235 GLEAN mRNA 16837 34725 0.738057 - . ID=CGI_10011828;  scaffold1249 494779 495082 scaffold1249 GLEAN mRNA 493149 497416 1 + . ID=CGI_10019076;  scaffold1264 68697 69025 scaffold1264 GLEAN mRNA 42236 82741 0.959625 + . ID=CGI_10007171;  scaffold1493 16031 16478 scaffold1493 GLEAN mRNA 11465 19397 1 + . ID=CGI_10006736;  scaffold1719 330025 330079 scaffold1719 GLEAN mRNA 328128 331031 1 - . ID=CGI_10014288;  scaffold1719 330285 330484 scaffold1719 GLEAN mRNA 328128 331031 1 - . ID=CGI_10014288;  scaffold1752 17551 17709 scaffold1752 GLEAN mRNA 8754 18177 0.400064 - . ID=CGI_10003565;  scaffold1752 17551 17709 scaffold1752 GLEAN mRNA 16055 20494 0.777485 + . ID=CGI_10003566;  scaffold1752 17916 17975 scaffold1752 GLEAN mRNA 8754 18177 0.400064 - . ID=CGI_10003565;  scaffold1752 17916 17975 scaffold1752 GLEAN mRNA 16055 20494 0.777485 + . ID=CGI_10003566;  scaffold193 65914 65965 scaffold193 GLEAN mRNA 59988 95620 0.99962 + . ID=CGI_10015222;  scaffold193 66130 66185 scaffold193 GLEAN mRNA 59988 95620 0.99962 + . ID=CGI_10015222;  scaffold193 66310 66371 scaffold193 GLEAN mRNA 59988 95620 0.99962 + . ID=CGI_10015222;  scaffold203 259584 259908 scaffold203 GLEAN mRNA 240078 263515 0.386443 + . ID=CGI_10021067;  scaffold247 141885 142442 scaffold247 GLEAN mRNA 139499 148449 0.991247 + . ID=CGI_10018592;  scaffold247 144031 144583 scaffold247 GLEAN mRNA 139499 148449 0.991247 + . ID=CGI_10018592;  scaffold308 50213 50645 scaffold308 GLEAN mRNA 48566 59085 0.73638 + . ID=CGI_10011004;  scaffold335 60010 60067 scaffold335 GLEAN mRNA 56499 76156 0.475758 + . ID=CGI_10017084;  scaffold335 60531 60693 scaffold335 GLEAN mRNA 56499 76156 0.475758 + . ID=CGI_10017084;  scaffold348 716142 716446 scaffold348 GLEAN mRNA 715076 738533 0.987905 - . ID=CGI_10023001;  scaffold372 176523 176578 scaffold372 GLEAN mRNA 173398 180404 1 - . ID=CGI_10009708;  scaffold372 176916 176971 scaffold372 GLEAN mRNA 173398 180404 1 - . ID=CGI_10009708;  scaffold372 177146 177197 scaffold372 GLEAN mRNA 173398 180404 1 - . ID=CGI_10009708;  scaffold394 555813 556110 scaffold394 GLEAN mRNA 549658 560561 0.811205 + . ID=CGI_10022093;  scaffold40282 64161 64479 scaffold40282 GLEAN mRNA 56091 83549 0.539379 + . ID=CGI_10004002;  scaffold42302 144946 145003 scaffold42302 GLEAN mRNA 139205 148407 0.999952 - . ID=CGI_10006575;  scaffold42302 145180 145356 scaffold42302 GLEAN mRNA 139205 148407 0.999952 - . ID=CGI_10006575;  scaffold43794 261331 261398 scaffold43794 GLEAN mRNA 257501 270559 0.726785 + . ID=CGI_10012420;  scaffold43794 261706 261891 scaffold43794 GLEAN mRNA 257501 270559 0.726785 + . ID=CGI_10012420;  scaffold457 43443 43495 scaffold457 GLEAN mRNA 36149 55409 0.303508 + . ID=CGI_10014527;  scaffold457 43703 43889 scaffold457 GLEAN mRNA 36149 55409 0.303508 + . ID=CGI_10014527;  scaffold481 577121 577537 scaffold481 GLEAN mRNA 564527 580694 0.999885 - . ID=CGI_10027225;  scaffold542 242268 242712 scaffold542 GLEAN mRNA 230390 250299 0.926479 - . ID=CGI_10014766;  scaffold542 247837 248022 scaffold542 GLEAN mRNA 230390 250299 0.926479 - . ID=CGI_10014766;  scaffold542 248198 248253 scaffold542 GLEAN mRNA 230390 250299 0.926479 - . ID=CGI_10014766;  scaffold579 198644 198702 scaffold579 GLEAN mRNA 193436 221323 0.863149 + . ID=CGI_10011264;  scaffold579 199044 199103 scaffold579 GLEAN mRNA 193436 221323 0.863149 + . ID=CGI_10011264;  scaffold579 199314 199373 scaffold579 GLEAN mRNA 193436 221323 0.863149 + . ID=CGI_10011264;  scaffold588 57559 57881 scaffold588 GLEAN mRNA 27826 75048 0.701659 + . ID=CGI_10016209;  scaffold942 185812 186123 scaffold942 GLEAN mRNA 167859 210633 0.24351 + . ID=CGI_10019155;           

track type=bedGraph name="2M_sig" description="2M_sig" visibility=full color=100,100,0 altColor=0,100,200 priority=20  scaffold1 163391 163444 -1.19635354862016  scaffold1 167390 167448 -1.34858424227208  scaffold1 177036 177092 -1.32513261026528  scaffold1 180263 180313 -1.59644601437398  scaffold1 184151 184202 -1.36802539236446  scaffold1 207852 207911 -1.4489540693628  scaffold1 221645 221697 -1.19168816975966  scaffold100 20261 20311 -1.38705592724581  scaffold100 43707 43766 -1.94554287545546  scaffold100 46611 46670 -1.2435587162076  scaffold100 46736 46797 -1.43149771518741  scaffold100 52475 52525 -1.25154235215334  scaffold100 53240 53290 -1.20752426644291  scaffold100 53370 53420 -1.28286420456278  scaffold100 99600 99660 -1.64937361688604  scaffold100 100215 100279 -1.78583379885635  scaffold100 239550 239610 -1.58756865327687  scaffold100 239920 239980 -1.48336190706549  scaffold100 240165 240223 -1.20454054682889  scaffold100 242056 242114 -1.20332332217023  scaffold100 242166 242222 -1.44656943837467  scaffold100 247943 247993 -1.4272470666282  scaffold100 250533 250586 1.72713841645018  scaffold100 362779 362836 1.24792813025432  scaffold100 377331 377381 -1.31760310939671  scaffold100 435766 435816 -1.30563674414056  scaffold100 437627 437684 1.26968497656438  scaffold100 439363 439415 1.8535900440036  scaffold100 458442 458498 1.33779652899652  scaffold100 461811 461861 -1.70172961914225  scaffold100 464334 464384 -1.34212321553077  scaffold100 474564 474614 -1.42748867932449  scaffold100 636974 637034 1.47573175617257  scaffold100 637084 637143 1.24656795811596  scaffold100 642110 642170 1.32784939242625  scaffold100 676066 676117 2.0744756115782  scaffold100 714882 714932 -1.65535183838333  scaffold100 715132 715182 -1.21696164835903  scaffold100 724829 724881 -1.88008540906678  scaffold100 755257 755308 -1.35255209139684  scaffold100 786576 786629 -1.39806522098838  scaffold100 788254 788310 -1.25599139882591  scaffold100 802660 802710 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Differential Methylation in the Kitchen  Finally re-getting around to examing our MBD-array data in the context of RNA-seq data. Briefly we have three oysters that were heat shocked with samples taken prior to, and following. An MBD-array experiment was carried out where for each oyster we have information on 50bp genome features (selected probes on the array) whether the region was hypomethylated, hypermethylated or did not change.  Following heat shock approximately 10k differentially methylated features (value >/= 1.8) were identified in all oysters. For all oysters a majority of the features were hypomethylated. Specifically, for oysters #2, #4, and #6 the number of hypomethylated features was 7224, 6560, and 7645, respectively. When only features were considered where at least 3 adjacent probes were also differentially methylated the number of features (merged) for oysters #2, #4, and #6 was 112, 58, and 62, respectively. A majority of these features were hypomethylated (108, 48, and 53, respectively)  Oyster | Hypo-methylated | Hyper-methylated | Hypo-3plus-merged | Hypo-3plus-merged  --- | --- | --- | --- | ---  2 | 7224 | 2803 | 108 | 4  4 | 6560 | 3587 | 48 | 10  6 | 7645 | 4044 | 53 | 9  The files with differentially methylated region (DMRs) as defined by value >/= 1.8 are name as `2014.07.02.6M_sig.bedGraph` and have the following format (ie `head` output)  ```  track type=bedGraph name="6M_sig" description="6M_sig" visibility=full color=100,100,0 altColor=0,100,200 priority=20  scaffold1 54599 54654 -1.38187662416007  scaffold1 162129 162191 1.85685479189849  scaffold1 163536 163586 -1.15032035523765  scaffold1 172654 172714 1.33561271440876  scaffold1 174287 174343 -1.62903936976887  scaffold1 178075 178128 1.42323539316231  scaffold1 178685 178740 1.30886296151914  scaffold1 184271 184330 -1.20699853451878  scaffold1 184661 184715 -1.61107459826899  ```  As part of the data provided by the core facility we also obtained genome feature files where there were three or more adjacent signficant probes. The naming structure is `2014.07.02.4M_Hypo_3plusAdjactentProbes.gff` with the file format:  ```  scaffold100 804406 804960  scaffold1018 367127 367319  scaffold1018 367618 367674  scaffold1077 401205 401534  scaffold12 244080 244376  scaffold1324 356489 356781  ```  To merge these adjacent features I ran `bedtools merge`. For example   ```  bedtools merge -d 100 -i \  ./data/2014.07.02.colson/genomeBrowserTracks/threeOrMoreAdjacentSigProbes/2014.07.02.2M_Hypo_3plusAdjactentProbes.gff \  > ./analyses/2M_3plusmerge_Hypo.gff   wc -l ./analyses/2M_3plusmerge_Hypo.gff   ```  These values are refered to in the above table as Hypo(Hyper)-3plus-merged and the file format:  ```  scaffold1737 485256 485565  scaffold1894 222981 223032  scaffold1894 223601 223653  scaffold1894 224777 224838  scaffold392 326519 326818  scaffold433 204898 205194  scaffold544 44676 44853  scaffold544 45028 45086  scaffold854 353009 353316  ```  filenames: `6M_3plusmerge_Hypo.bed`