this is for holding javascript data
Steven Roberts added bed graphs
almost 9 years ago
Commit id: 886f906654319f486ee3ea4b7d1758b6891bd60b
deletions | additions
diff --git a/genome-feature-tracks/2014.07.02.2M_sig.bedGraph b/genome-feature-tracks/2014.07.02.2M_sig.bedGraph
new file mode 100755
index 0000000..22397b9
--- /dev/null
+++ b/genome-feature-tracks/2014.07.02.2M_sig.bedGraph
...
track type=bedGraph name="2M_sig" description="2M_sig" visibility=full color=100,100,0 altColor=0,100,200 priority=20
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diff --git a/genome-feature-tracks/2014.07.02.4M_sig.bedGraph b/genome-feature-tracks/2014.07.02.4M_sig.bedGraph
new file mode 100755
index 0000000..9db2f9d
--- /dev/null
+++ b/genome-feature-tracks/2014.07.02.4M_sig.bedGraph
...
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diff --git a/genome-feature-tracks/2014.07.02.6M_sig.bedGraph b/genome-feature-tracks/2014.07.02.6M_sig.bedGraph
new file mode 100755
index 0000000..0ae6caa
--- /dev/null
+++ b/genome-feature-tracks/2014.07.02.6M_sig.bedGraph
...
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diff --git a/methods-rna2.md b/methods-rna2.md
index ff75f73..58c3f84 100644
--- a/methods-rna2.md
+++ b/methods-rna2.md
...
#methods-RNAseq
Total RNA was extracted using Tri-Reagent per manufacturer’s instruction. Potential DNA carry-over was removed from extracted RNA using the Turbo DNA-free treatment according to the manufacturer's instructions (Ambion).
Libraries Messenger RNA (mRNA) enrichment, library preparation, and sequencing was performed by GENEWIZ. Poly(A) enrichment was performed using NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Illumina sequencing libraries were prepared
using with the
Illumina TruSeq NEBNext® Ultra™ RNA
Sample Preparation kit according to Library Prep Kit for Illumina® (New England Biolabs) and each library was uniquely barcoded with the
manufacturer’s protocol (including bar-coding NEBNext® Multiplex Oligos for
multiplexing). Samples Illumina® (Index Primers Set 1) (New England Biolabs). All six libraries were multiplexed
where three samples were and run
in one on a single lane
for Illumina Hiseq 100 bp paired end sequencing. on a HiSeq2500 (Illumina), with a read length of 100bp (single end).
RNA-seq analysis was carried out using the TopHat2 suite in the iPlant Collaborative Discovery Environment. Specifically TopHat2-SE was used to align the six libraries (3 individuals, pre and post heat shock) to the _Crassostrea gigas_ genome.
diff --git a/results-array.md b/results-array.md
index 2c7e365..1194ad5 100644
--- a/results-array.md
+++ b/results-array.md
...
#results-array
In direct comparison of methylation for an individual oyster prior to and following heat shock approximately 10k differentially methylated features were
identified. identified as defined by >/= 1.8 difference. For all oysters a majority of the features were hypomethylated. Specifically, for oysters #2, #4, and #6 the number of hypomethylated features was 7224, 6560, and 7645, respectively. When only features were considered where at least 3 adjacent probes were also differentially methylated the number of features
(merged) (adjacent probes merged) for oysters #2, #4, and #6 was 112, 58, and 62, respectively. A majority of these features were hypomethylated (108, 48, and 53, respectively)
Oyster | Hypo-probes | Hyper-probes | Hypo-3plus-merged | Hypo-3plus-merged
--- | --- | --- | --- | ---
...
6 | 7645 | 4044 | 53 | 9
In order to examine regions that were differentially methylated across all three
oysters oysters, features were identified where there were at least 3 adjacent probes with
signficantly significantly differentially methylated. A total of 111 features were identified as hypomethylated and 54 features as hypermethylated when differentially methylated probe with 100bp were merged.