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#methods-experiment  Adult oysters were anesthetized by held overnight in tanks (6L) with MgCl2 (50g/l). Gill and mantle Mantle  tissue was taken and placed at -80C and oysters placed back into seawater. Oysters were subjected to a 40C, 1 hour acute heat shock 7 days later. Tissue samples (gill and mantle) was were  taken immediately following heat shock exposure placed at -80C. > likely all analysis is on mantle d