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#methods-experiment  Adult oysters n=3 (191.93 g+/- 56.45) (Mean +/- SD)  were anesthetized byheld  overnight holding  in tanks (6L) with MgCl2 (50g/l). Mantle The following day, mantle  tissue was taken and placed at -80C and oysters placed back into seawater. Oysters After 7 days, oysters  were subjected to a 40C, 1 hour acute heat shock 7 days later. shock.  Tissue samples were taken immediately following heat shock exposure placed at -80C.