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\section{Results and Discussion}  \textbf{Temporal microbial succession during C and C-cycling dynamics  degradation.} With the rapid advancement and declining costs of high throughput sequencing, it has become increasingly easy to investigate microbial communities. In this study, we couple stable-isotope probing with 454 pyrosequencing in order to better understand organic matter decomposition dynamics as a function of soil microbial community C utilization. We ran a temporal series of parallel microcosms and measured the changes in the microbial community as result of the addition of a complex C mixture using 454 pyrosequencing of the bulk microbial community and fractions from CsCl gradient fractionation (Fig. S1). Overall, temporal changes in microbial community composition are consistent with C decomposition being accompanied by a microbial community succession. Analysis of the sequenced bulk community DNA demonstrates Proteobacteria (26-35\%), Actinobacteria (19-26\%), and Acidobacteria (12-21\%) as the most dominant phyla throughout the duration of the experiment. This is consistent with previous observations \cite{Goldfarb_2011,Fierer_2007,Rui_2009,Fierer_2012}. We found trends of Proteobacteria and Actinobacteria decreasing and Acidobacteria increasing as C availability declines (TableS1). This is congruent with findings in soils sampled from a wide range of ecosystems in the US \cite{Fierer_2007}. At days 1, 3, and 7 the bulk community was composed of $\sim$12-18\% Bacteriodetes and Firmicutes (combined). At days 14 and 30, these phyla declined to a combined 7-9\% of the whole community accompanied with an increase in Planctomycetes, Verrucomicrobia, and Chloroflexi (2-3\% at day 1 to 5-7\% at day 30). There has been conflicting evidence about the correlation of Bacteriodetes abundance with C availability \cite{Fierer_2007,Rui_2009,Sharp_2000,L_pez_Lozano_2013,Bastian_2009}. Our bulk data demonstrates a positive correlation between labile C availability and Bacteroidetes abundance. Additionally, the abundance of Planctomycetes, Verrucomicrobia, and Chloroflexi at later stages of decomposition are in accord with findings in wheat straw degradation \cite{Bastian_2009}. The rank abundance (RA) of the community depicts transitions we observe in high ranking phyla abundance beginning at day 7 (Fig S2A). Despite the fluctuations we observe at the phylum level, the biological variability observed over time is low (FigS2B) demonstrating community stability.