Chuck Pepe-Ranney edited Disscussion.tex  over 9 years ago

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\textbf{Cellulose degraders identified from undescribed lineages and cosmoplitan  soil taxa for which functional attributes are not established}  \textit{Verrucomicrobia} Goldfarb paper, Fierer papers are ubiquitous in soil worldwide CITE. \textit{Verrucomicrobia}   can constitude as much as XX\% of 16S rRNA gene sequences in soil clone libraries CITE and  have been shown to represent XX\% of soil rRNA CITE. Many \texit{Verrucomicrobia} cultivars  have been established in the last decade CITE FIGURE. Genomic analyses and physiological   profiling of \textit{Verrucomicrobia} isolates have revealed methanotrophy CITE and diazotrophy   within \textit{Verrucomicria} CITE. The genetic capacity to degrade cellulose  has also been discoverd in \textit{Verrucomicrobia} \citep{Wertz_2011}. Although, we have  learned many functional roles of \textit{Verrucomicrobia} in the environment, the function and/or  importatance of soil \textit{Verrucomicrobia} in C-cycling is largely unknown. None of the   putative verrucomicrobial cellulose degraders identified in this experiment are closely related  to names cultivars and only XX\% of all verrucomicrobial OTUs found in this study share at lease  97\% sequence identity with isolates. HR-SIP identifies key players in soil C-cycling that  represent important targets for single cell and community genomics studies CITE CITE.   \textit{Verrucomicrobia} lineages, given there ubiquity and abundance in soil, may play   hugely influential roles in global soil cellulose respiration.  \textit{Chloroflexi} Banfield paper