Chuck Pepe-Ranney deleted file results_p13.tex  over 9 years ago

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\subsection{Cellulose degrader DNA shifts further along the BD gradient upon  $^{13}$C incorporation than xylose degrader DNA}   Cellulose responders exhibited a greater shift in BD than xylose responders in  response to isotope incorporation (Figure~\ref{fig:shift}, p-value  1.86e$^{-06}$). $^{13}$C-cellulose responders shifted on average 0.0163 g/mL  (sd 0.0094) whereas xylose responders shifted on average 0.0097 (sd  0.0094). For reference, 100\% $^{13}$C DNA shifts X.XX g/mL  relative to the BD of its $^{12}$C counterpart. DNA BD increases  as its ratio of $^{13}$C to $^{12}$C increases. An organism that only  assimilates C into DNA from a $^{13}$C isotopically labeled source, will have a  greater $^{13}$C:$^{12}$C ratio in its DNA than an organism utilizing a mixture  of isotopically labeled and unlabeled C sources. Upon labeling, DNA from an  organism that incorporates exclusively $^{13}$C will increase in buoyant density  more than DNA from an organism that does not exclusively utilize  isotopically labeled C. Therefore the magnitude DNA buoyant density shifts  indicate substrate specificity given our experimental design as only one  substrate was labeled in each amendment. We measured density shift  as the change in an OTU's density profile center of mass between corresponding  contol and labeled gradients. Density shifts, however, should not be evaluated  on an individual OTU basis as a small number of density shifts are observed for  each OTU and the variance of the density shift metric at the level of  individual OTUs is unknown. It is therefore more informative to compare density  shifts among substrate responder groups. Further, density shifts are based on  relative abundance profiles and would be theoretically muted in comparison to  density shifts based on absolute abundance profiles and should be interpreted  with this transformation in mind. It should also be noted that there was  overlap in observed density shifts between $^{13}$C-cellulose and  $^{13}$C-xylose responder groups suggesting that although cellulose degraders  are generally more substrate specific than xylose utilizers, some cellulose  degraders show less substrate specificity for cellulose than some xylose  utilizers for xylose (Figure~\ref{fig:shift}), and, each responder group  exhibits a range of substrate specificites (Figure~\ref{fig:shift}).