Ashley Campbell edited Results_and_Discussion.tex  over 9 years ago

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At day 1 (d1), 57\% of xylose responsive OTUs belong to Firmicutes (Paenibacillaceae, Planococcaceae, and Bacillaceae) and the remaining 43\% of responders were comprised of 19\% Bacteroidetes (Flavobacteriaceae), 14\% Proteobacteria (Enterobacteriaceae, Comamonadaceae, and uncultured Gammaproteobacteria), and 10\% Actinobacteria (Micrococcaceae and Microbacteriaceae) (\href{https://www.authorea.com/users/3537/articles/3612/master/file/figures/l2fc_fig1/l2fc_fig.pdf}{Fig. 2}). Of the xylose responders detected at day 3 (d3), Firmicutes responders decreased to 8\% (from 12 OTUs to 1) and an increase in Bacteroidetes (67\%) and Proteobacteria (25\%) from d1. Day 7 (d7) responders were 50\% Actinobacteria, 35\% Proteobacteria, and 5\% of each Bacteroidetes, Firmicutes, and Planctomycetes. A substantial amount (75\%) of xylose responders for day 7 had not previously been identified as responders at earlier time points which attests to the wide number of taxa able to use xylose. Each of the 10 Actinobacteria responders, the most dominant response group (50\%) at day 7, belonged to a different family making it the phylum with the most wide-spread use of xylose. However, it should be noted that they were confined within two Actinobacterial Orders; Frankiales and Micrococcales.   We observe dynamic changes in \textsuperscript{13}C-xylose assimilation with time; dominant xylose responders shift from Firmicutes (d1) to Bacteroidetes (d3) then finally Actinobacteria (d7). At any given time soils harbor microorganisms at varying degrees of dormancy depending on nutrient availability \cite{Jones_2010}. The sudden addition of our complex C mixture would most certainly prompt dormant and non-dormant microbes back into metabolic activity, with those exhibiting higher rRNA operon copy numbers responding the fastest. The responders identified at d1 for xylose utilization have all been noted for exhibiting some form of dormancy strategy \cite{Jones_2010, Mulyukin_2009, Darcy_2011, Sachidanandham_2008, Finkel_2006, Rittershaus_2013, Tada_2013, Lay_2013}, though the only spore-forming responders at d1 are Firmicutes. Additionally, d1 responders exhibit 6-14 rRNA operon copies with the exception of the Betaproteobacteria Comamonadaceae and the Actinobacterial OTUs which exhibit 1-2 copies according to representative taxa in the rrnDB v. 3.1.227 \cite{18948294,11125085}. With the exception of the single Firmicutes (Paenibacillaceae), responders on d3 possess 3-6 rRNA operon copies, less than responders on d1. Similar to d3, 85\% of d7 responders exhibit between 1-5 rRNA operon copies with the remaining 15\% (Flavobacteriaceae, Enterobacteriaceae, and Paenibacillaceae) containing between 6-14 rRNA operon copies. Paenibacillus (100\% identity) was the only OTU to be identified at every time point (up to day 30) as a xylose responder. This result suggests that large numbers of cells from Paenibacillus sporulated after \textsuperscript{13}C-labeling of their DNA and that these spores remained throughout the experiment. It was the the second most enriched xylose responder, log\textsubscript{2}fold log$_{2}$fold  change (l2fc) of 3.5, measured in the time series second only to a Gammaproteobacteria (Xanthomonadaceae; l2fc = 3.7). \textit{Cellulose}. In contrast with xylose responders, there were only three \textsuperscript{13}C-cellulose responders detected within the first 7 days of incubation and 46 for days 14 and 30. An average of 16\% of the \textsuperscript{13}C-cellulose added was respired within the first 7 days, 38\% by day 14 (d14), and 60\% by day 30 (d30). The earliest responders detected for \textsuperscript{13}C-cellulose assimilation were a canonical cellulose degrader, Cellvibrio, in Proteobacteria and a novel clade in Chloroflexi (\href{https://www.authorea.com/users/3537/articles/3612/master/file/figures/l2fc_fig1/l2fc_fig.pdf}{Figs. 2}, \href{https://authorea.com/users/3537/articles/8459/master/file/figures/l2fc_fig_pVal/l2fc_fig_pVal.png}{& S4}). At day 14, 55\% of the responders belong to Proteobacteria (65\% Alpha-, 23\% Gamma-, and 12\% Beta-), 13\% Chloroflexi, 13\% Planctomycetes, 10\% Verrucomicrobia, 6\% Actinobacteria, and 3\% Cyanobacteria.