Ashley Campbell edited Results_and_Discussion.tex  over 9 years ago

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One of the most interesting findings is a single Cyanobacterial responder OTU, which exhibit the third greatest enrichment measured (l2fc = 3.35) in response to \textsuperscript{13}C-cellulose assimilation. This OTU falls into the recently described candidate phylum Melainabacteria \cite{Di_Rienzi_2013}, although its phylogenetic position is debated \cite{Soo_2014}. More importantly, the catalog of metabolic capabilities associated with Cyanobacteria are quickly expanding \cite{Di_Rienzi_2013, Soo_2014}. Our findings provide evidence of cellulose degradation for Melainabacteria, supporting hypothesized polysaccharide degradation suggested by genomic analysis \cite{Di_Rienzi_2013}.   Proteobacteria represent 47\% of all cellulose responders identified. Of those, Cellvibrio accounted for 5\% of all Proteobacterial responders detected (\href{https://www.authorea.com/users/3537/articles/3612/master/file/figures/l2fc_fig1/l2fc_fig.pdf}{Figs. 2}, \href{https://authorea.com/users/3537/articles/8459/master/file/figures/l2fc_fig_pVal/l2fc_fig_pVal.png}{S4}, \href{https://authorea.com/users/3537/articles/8459/master/file/figures/cellulose_resp_profiles/cellulose_resp_profiles.png}{S6}). Cellvibrio was one of the first identified cellulose degrading bacteria and was originally described by Winogradsky in 1929 who named it for its cellulose degrading abilities(Bergey's ISBN 9780387241449). Details about max density shift (need to talk to Chuck because this data is not easy to acquire). Other prominent cellulose degrading Proteobacteria, Stenotrophomonas and Devosia have previous previously  been demonstrated in degrading cellulose \cite{Trujillo_Cabrera_2012, Verastegui_2014}. Brevundimonas has not previously been identified as a cellulose degrader, but has been show to degrade cellouronic acid, an oxidized form of cellulose \cite{Tavernier_2008}. There were 6 shared responders among all unique responders identified in both the xylose and cellulose treatments (n = 72); Stenotrophomonas, Planctomyces, two Rhizobiaceae, Comamonadaceae, and Cellvibrio. Of these, Stenotrophomonas and Comamonadaceae are the only taxa that are among the top ten l2fc responses measured in both treatments. On the other hand, the only shared responder that is not among the top ten responders for either the cellulose or xylose treatment is Rhizobiaceae. Two of the shared responders corresponded in time between the two treatments (supplemental table); Cellvibrio (d3) and Planctomyces (d14).