Ashley Campbell edited Results & Discussion.tex  almost 10 years ago

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Noteably we also identified a previously undescribed clade of Chloroflexi that exhibit a high substrate specificity based on a density shift peak at between 0.03-0.04 gmL\textsuperscript{-1}. We saw no other cellulose utilization in Chloroflexi outside of this clade. This phylum has previously been demonstrated to utilize cellulose (Goldfarb)   \textbf{Carbon substrate utilization is inconsistent within phylum.} More often than not we see ecological functionality assigned at the phylum level (refs). More specifically, it has been proposed that the microbial community functionality responsible for soil C cycling appear at the level of phlya rather than species/genera \cite{Schimel_2012}.  However, based on our evidence of clade or single taxa level responses for xylose and cellulose utilization, assigning phylum level functionality is not an accurate depiction of substrate soil C  utilization. Phylum level assignment conventions could in part be due to limitations in finer scale taxonomic identifications or to lack of sequencing resolution as seen here with many responders present in the rarer ranks (Figure 3B). Whole phylum responses were not detected for xylose or cellulose yet utilization of these substrates spanned many phylogenetically diverse groups. However, substrate utilization within each phylum was demonstrated at the clade or single taxa level. Cellulose degradation has previously been noted for spanning eight phyla in a study of forest soils amended with different carbon substrates, but was limited to a small number of taxa within each of those phyla \cite{Goldfarb_2011}. It has previously been suggested that all taxa within a phylum are unlikely to share ecological characteristics \cite{Fierer_2007}, and furthermore, within a species population \cite{Choudoir_2012}\cite{Preheim_2011}\cite{Hunt_2008}. To portray the response of a few OTUs or clades as a phylum level response would be overreaching.  For both xylose and cellulose, whole phylum responses were not detected yet utilization of these substrates spanned many phylogenetically diverse groups. Although substrate utilization within each phylum was either clade or single taxa specific. Cellulose degradation has previously been noted for spanning eight phyla in a study of forest soils amended with different carbon substrates, however, utilization was limited to a small number of taxa within each of those phyla \cite{Goldfarb_2011}. It has previously been suggested alluded that all taxa within a phylum are unlikely to share ecological characteristics and the data presented here is in support of that \cite{Fierer_2007}. To portray the response of a few OTUs or clades as a phylum level response would be overreaching.   "In their effects on soil C cycling, the influences of microbial com- munities appear to be associated with life history patterns that are deeply rooted in microbial phylogeny – functional groups appear at the level of families or phyla rather than species or genera. "\cite{Schimel_2012}  Although it is imortant to note that within species   For example, using an approach to map habitat traits onto microbial phylogeny, ecological popu- lations within coastal Vibrio isolates can be predicted based on seasonal occurrence and particulate size fractionation (Hunt et al., 2008; Preheim et al., 2011).