Ashley Campbell edited Results & Discussion.tex  almost 10 years ago

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    Boast about what this technique can do/ \textbf{Conclusions.} We have demonstrated  how it's added to our field.  Discuss the short-comings  While next generation sequencing-enabled SIP gives a taxa level resolution for substrate utilization. Using this technique,  we arecurrently  able to resolve highly responsive discrete  OTUs which has provided that would otherwise be missed using bulk community sequencing. Additionally, this technique provides  greater taxonomic resolution than previous techniques (cloning, TRFLP), TRFLP, ARISA) used to determine substrate utilizing community members. While we are currently able to resolve highly responsive OTUs,  the next step is to find better ways to resolve taxa that are partially responsive which we are not currently  able to differentiate from noise. noise with confidence.  Yet, given the ability to resolve partially responsive taxa, the ecology would still be difficult to discern. For example, a generalist utilizing many substrates including the 13C-labeled substrate may exhibit the same partial labeling that a specialist utilizing both the 13C-substrate and the same substrate (unlabeled) that is inherent in the soil. Additionally, partially labeled taxa could be further down the trophic cascade including predators or secondary consumers of waste products from primary consumer microbes that were highly labeled. Our study is consistent with carbon degradative succession has previously been demonstrated (ref). We demonstrate a rapid decrease in the labile carbon, xylose, confirmed by it’s 13C label incorporation into the microbial community DNA during the first 7 days of the experiment, after which, the label is not detectable in the DNA. Subsequently our data demonstrates a slow degradation of the more recalcitrant, polymeric carbon demonstrated by 13C-cellulose label incorporation into the microbial community DNA at 14 and 30 days. We did not observe the 13C-cellulose signal leave the DNA within the time limits, 30 days, of our experiment. This degradation succession is also confirmed by isotopic analysis of the soil from the microcosms. NMDS demonstrates microbial succession and based on xylose and cellulose treatments separating away from the control, but in opposing directions indicating different microbial community members are responsible for C degradation of the two substrates.