Ashley Campbell edited Methods.tex  over 10 years ago

Commit id: b035da37bed6d115527ffdd9b6ffc79fe3dafb53

deletions | additions      

       

\textbf{Microcosm}  A subset of soil was dried at 105°C overnight to determine soil moisture content. Microcosms (35 total) were created with the equivalent of ten grams dry soil weight of the sieved soil in a 250mL Erlenmeyer flask capped with a butyl rubber stopper to preventing drying of soils. Microcosms were kept at 25°C for 2 weeks for bottle conditioning (better phrasing: pre-incubation?) and stoppers were removed for 10min every 3 days to prevent anoxia of soils. A complex carbon mixture (38\% cellulose, 23\% lignin, 20\% xylose, 3\% arabinose, 1\% galactose, 1\% glucose, 0.5\% mannose) was designed based on switch grass biomass composition. This complex carbon mixture was added to microcosms along with an amino acid (in-house made replica of teknova Cat#C0705) and basal salt mixture (Murashige and Skoog, Sigma M5524) at 52.6mg^{-1}10g soil; representative of natural concentrations. Cellulose (20mg) and lignin (12mg) were sprinkled over the surface of the soil in each microcosm as dry additions due to their insolubility to ensure same concentration in every flask. The remaining carbons, amino acids, and basal salts were added by pipetting evenly over soil surface as a liquid addition until 50% 50\%  water holding capacity is achieved. Three parallel treatments were performed; (1) control, all \textsuperscript{12}C-carbons, (2) cellulose, all \textsuperscript{12}C-carbons except \textsuperscript{13}C-cellulose, (3) xylose, all \textsuperscript{12}C-carbons except \textsuperscript{13}C-xylose. (Microcosms remained stoppered throughout incubation, except for every third day when flasks were flushed with fresh air for 2 minutes. 50% water holding capacity measured.