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...
approach that expands upon previous nucleic acid SIP methods in three dimensions:
1) temporally, we sample isotopically labeled substrate amended microcosms at multiple
time points; 2) spacially, we assay more fractions along the CsCl gradients; and 3),
bioinformatically, we interrogate taxa at the level
or of OTU for isotope incorporation
employing cutting edge statistics for assessing differential abundance in microbiome
datasets.
diff --git a/Results.tex b/Results.tex
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...
addition has likely been stabilized by assimilation into microbial biomass
and/or microbial conversion into other forms of organic matter, though it is
possible that some $^{13}$C-xylose remains unavailable to microbes due to
abiotic interactions in soil \citep{Kalbitz_2000}.
All xylose
responders were first responsive in first 7 incubation days.
At day 1, 84\% of xylose responsive OTUs belong to Firmicutes, 11\% to
\textit{Proteobacteria} and 5\% to \textit{Bacteroidetes}. At day 3,
...
``OTU.183'' (closest LTP BLAST hit, \textit{Chitinophaca sp.}, 89.5\% sequence
identity). OTU.183 shares high sequence identity with environmental clones
derived from rhizosphere samples (accession AM158371, unpublished) and the skin
microbiome (accession JF219881,
CITE). \citet{Kong_2012}). Other \textit{Bacteroidetes} responders
share high sequence identities with canonical soil genera including
\textit{Dyadobacer}, \textit{Solibius} and \textit{Terrimonas}. Six of the 8
\textit{Actinobacteria} $^{13}$C-xylose responders are in the
...
than xylose responders at days 3 and 7, and, Xylose responders have more rRNA operon copy numbers
than cellulose responders.}
Estimated rRNA operon genome copy numbers per $^{13}$C-xylose responder OTU
genome and
and day of first response are correlated (p-value 2.02e$^{-15}$,
Figure~\ref{fig:copy}). $^{13}$C-xylose responder rRNA operon geneome copy
number is inversely related to time; that is, OTUs that first respond at later
time points have fewer estimated rRNA operons per genome than OTUs that first
...
responder is fairly abundant in the bulk samples (``OTU.5'',
Table~\ref{tab:cell}) with a mean bulk rank of 13 (\textit{i.e.} on average the
13th most abundant OTU) and a $^{13}$C-xylose responder (``OTU.1040'',
Table~\ref{tab:xyl}) has a mean
abundance in bulk relative abundance in
bulk samples
of 2.85e$^{-05}$. Only one substrate responder ($^{13}$C-cellulose) was not
found in any bulk samples ("OTU.862", Table~\ref{tab:cell}). Of the top 10
responders sorted by descending mean rank (essentially the 10 most
abundandant abundant
responders in the bulk samples), 8 are $^{13}$C-xylose responders and 5 of
these 8 have mean ranks less than 10 in bulk samples.
\subsection{Variation in bulk soil DNA microbial community structure is significantly less
diff --git a/bibliography/biblio.bib b/bibliography/biblio.bib
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...
title = {{{UniFrac}: a New Phylogenetic Method for Comparing Microbial Communities}},
journal = {Applied and Environmental Microbiology},
}
@article{Kong_2012,
doi = {10.1101/gr.131029.111},
url = {http://dx.doi.org/10.1101/gr.131029.111},
year = {2012},
month = {feb},
publisher = {Cold Spring Harbor Laboratory Press},
volume = {22},
number = {5},
pages = {850--859},
author = {H. H. Kong and J. Oh and C. Deming and S. Conlan and E. A. Grice and M. A. Beatson and E. Nomicos and E. C. Polley and H. D. Komarow and P. R. Murray and M. L. Turner and J. A. Segre},
title = {{Temporal shifts in the skin microbiome associated with disease flares and treatment in children with atopic dermatitis}},
journal = {Genome Research},
}